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Human MMP-9 Antibody

Catalog #: AB911
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AB911
MMP‑9 in MMP‑9 transfected NS0 Mouse Cell Line.
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Product Details
Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MMP-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP‑9 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -10,
-12 and -13 is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MMP‑9 Western Blot Standard (Catalog # WBC018)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑9 (Catalog # 911-MP), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry MMP-9 antibody in MMP-9 transfected NS0 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

MMP‑9 in MMP‑9 transfected NS0 Mouse Cell Line. MMP‑9 was detected in immersion fixed MMP-9 transfected NS0 mouse myeloma cell line using Goat Anti-Human MMP‑9 Polyclonal Antibody (Catalog # AB911) at 5 µg/mL for 3 hours at room temperature. Cells were stained (red) and counterstained (green). Specific labeling was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry MMP-9 antibody in Human Liver Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑9 in Human Liver Cancer Tissue. MMP-9 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human MMP-9 Polyclonal Antibody (Catalog # AB911) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-9

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Long Name
Matrix Metalloproteinase 9
Entrez Gene IDs
4318 (Human); 17395 (Mouse); 81687 (Rat); 102117693 (Cynomolgus Monkey)
Alternate Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase

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Citations for Human MMP-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. C3d Elicits Neutrophil Degranulation and Decreases Endothelial Cell Migration, with Implications for Patients with Alpha-1 Antitrypsin Deficiency
    Authors: LT Fee, D Gogoi, ME O'Brien, E McHugh, M Casey, C Gough, M Murphy, AM Hopkins, TP Carroll, NG McElvaney, EP Reeves
    Biomedicines, 2021-12-16;9(12):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Inhibition of matrix metalloproteinase-9 secretion by dimethyl sulfoxide and cyclic adenosine monophosphate in human monocytes
    Authors: Darcy R Denner, Maria LD Udan-Johns, Michael R Nichols
    World Journal of Biological Chemistry
  3. Distribution of Matrix Metalloproteinases in Human Atherosclerotic Carotid Plaques and Their Production by Smooth Muscle Cells and Macrophage Subsets
    Authors: Nynke A. Jager, Bastiaan M. Wallis de Vries, Jan-Luuk Hillebrands, Niels J. Harlaar, René A. Tio, Riemer H. J. A. Slart et al.
    Molecular Imaging and Biology
  4. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015-07-24;195(5):2429-41.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  5. Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
    Authors: Pegorier S, Wagner LA, Gleich GJ, Pretolani M
    J. Immunol., 2006-10-01;177(7):4861-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation.
    Authors: Rouy D, Ernens I, Jeanty C, Wagner DR
    Anal. Biochem., 2005-03-15;338(2):294-8.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot

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