Human Mimecan Antibody Summary
Ala20-Phe298
Accession # P20774
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Human Mimecan by Western Blot. Western blot shows lysates of human aorta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Mimecan at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Mimecan by Simple WesternTM. Simple Western lane view shows lysates of human heart (aorta) tissue, loaded at 0.2 mg/mL. A specific band was detected for Mimecan at approximately 44 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Mimecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2660) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mimecan
Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine‑rich repeats in the central domain, which is flanked by N- and C-terminal cysteine-rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C-terminal amino acid residues) and Mimecan (containing 223 most C-terminal residues). Human Mimecan shares approximately 85% amino acid sequence homology with the mouse or rat Mimecan.
Product Datasheets
Citation for Human Mimecan Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Osteoglycin-induced VEGF Inhibition Enhances T Lymphocytes Infiltrating in Colorectal Cancer
Authors: X Hu, YQ Li, QG Li, YL Ma, JJ Peng, SJ Cai
EBioMedicine, 2018-07-21;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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