Human MICB PE-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of MICB antibody in K562 Human Cell Line antibody by Flow Cytometry. Detection of MICB antibody in K562 Human Cell Line antibody by Flow Cytometry.](https://resources.rndsystems.com/images/datasheets/antibody/MICB_FAB1599P_Flow_Cytometry_13952.jpg)
Detection of MICB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB PE-conjugated Monoclonal Antibody (Catalog # FAB1599P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
![MICB Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. MICB Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.](https://resources.rndsystems.com/images/datasheets/antibody/MICB_FAB1599P_Knockout_Validated_23008.jpg)
MICB Specificity is Shown by Flow Cytometry in Knockout Cell Line. MICB knockout K562 human myelogenous leukemia cell line was stained with PE-conjugated Mouse Anti-Human MICB Monoclonal Antibody (Catalog # FAB1599P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). No staining in the MICB knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: MICB
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).
- Groh, V. et al. (2001) Nature Immunol. 2:255.
- Stephens, H. (2001) Trends Immunol. 22:378.
- Bauer, S. et al. (1999) Science 285:727.
- Groh, V. et al. (2002) Nature 419:734.
- Steinle, A. et al. (2001) Immunogenetics 53:279.
- Pende, D. et al. (2002) Cancer Res. 62:6178.
- Salih, H. et al. (2003) Blood 102:1389.
Product Datasheets
Citations for Human MICB PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Resveratrol promotes MICA/B expression and natural killer cell lysis of breast cancer cells by suppressing c-Myc/miR-17 pathway
Authors: Jie Pan, Jiaying Shen, Wengong Si, Chengyong Du, Danni Chen, Liang Xu et al.
Oncotarget
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NKG2D- and T-cell receptor-dependent lysis of malignant glioma cell lines by human gamma δ T cells: Modulation by temozolomide and A disintegrin and metalloproteases 10 and 17 inhibitors
Authors: Guranda Chitadze, Marcus Lettau, Stefanie Luecke, Ting Wang, Ottmar Janssen, Daniel Fürst et al.
OncoImmunology
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Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer
Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS;
Oncology letters
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Engineering of immune checkpoints B7-H3 and CD155 enhances immune compatibility of MHC-I-/- iPSCs for beta cell replacement
Authors: R Chimienti, T Baccega, S Torchio, F Manenti, S Pellegrini, A Cospito, A Amabile, MT Lombardo, P Monti, V Sordi, A Lombardo, M Malnati, L Piemonti
Cell Reports, 2022-09-27;40(13):111423.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
Authors: S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli
Cell Death & Disease, 2021-09-04;12(9):836.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis
Authors: Y Zhu, J Xie, J Shi
Bmc Biology, 2021-07-30;19(1):140.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
J Immunol, 2014-04-30;192(11):5332-42.
Species: Human
Sample Types: Whole Blood
Applications: Flow Cytometry -
Plasmodium falciparum-infected erythrocytes induce granzyme B by NK cells through expression of host-Hsp70.
Authors: Bottger E, Multhoff G, Kun JF, Esen M
PLoS ONE, 2012-03-15;7(3):e33774.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Mapping the biogenesis of forward programmed megakaryocytes from induced pluripotent stem cells
Authors: Moyra L, Arash S, Susanne B et al.
Sciences Advances
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