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Human MICA Antibody

Catalog #: MAB1300
39 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1300-SP
MAB1300-100
MAB1300-500
Detection of MICA in K562 Human Cell Line by Flow Cytometry.
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Citations (39)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MICA Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MICB is observed.
Source
Monoclonal Mouse IgG2B Clone # 159227
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human MICA
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.02-0.06 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human MICA Fc Chimera (Catalog # 1300-MA) to immobilized Recombinant Human NKG2D Fc Chimera (Catalog # 1299-NK) coated at 2 µg/mL (100 µL/well). At 0.4 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MICA antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MICA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated MICA Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

MICA Specificity is Shown by Flow Cytometry in Knockout Cell Line. MICA knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the MICA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human MICA by Flow Cytometry View Larger

Detection of Human MICA by Flow Cytometry Multiple receptors and ligands are involved in NK cell-mediated lysis of activated CD4+ T cells.Role of (A) activating and (B) inhibitory NK receptors in NK cell degranulation. Left column: representative histograms (of n≥3) for surface expression of ligands on activated (thick black line) and resting CD4+ T cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated CD4+ T) and filled histogram (resting CD4+ T). Middle- and right column: NK and CD4+ T cells were activated for 4 days in vitro as described, and co-cultured for 4 hours with 10 ug/mL mAb (or relevant isotype-matched control Ig). Degranulation is shown for CD56dim (middle column) and CD56bright (right column) NK cells. Representative histograms of surface expression of receptors on activated (thick black line) and resting NK cells (thin black line). Isotype-matched control Ig are represented by dashed line (activated NK) and filled histogram (resting NK). * P<0.05, ** P<0.005, *** P<0.001. (C) Sorted IL-2-activated CD56dim and CD56bright NK cells were co-cultured with 51Cr-labeled activated CD4+ T cells in a 51Cr-release assay with human IgG4 isotype control (•) or anti-NKG2A mAb (○). Data represents n = 3 experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22384114), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICA

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

References
  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. NKG2D and its Ligands (2002) http://www.RnDSystems.com
Long Name
MHC Class I-related Protein A
Entrez Gene IDs
100507436 (Human)
Alternate Names
FLJ60820; MGC111087; MICA; PERB11.1

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Citations for Human MICA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Intragenic transcriptional interference regulates the human immune ligand MICA
    Authors: Da Lin, Thomas K Hiron, Christopher A O'Callaghan
    The EMBO Journal
  2. Antitumor effects of NK cells expanded by activation pre?processing of autologous feeder cells before irradiation in colorectal cancer
    Authors: Koh, EK;Lee, HR;Son, WC;Park, GY;Bae, J;Park, YS;
    Oncology letters
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
    Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
    Nature Communications, 2022-03-25;13(1):1606.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
    Authors: S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli
    Cell Death & Disease, 2021-09-04;12(9):836.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing
    Authors: E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim
    PloS Pathogens, 2021-05-03;17(5):e1008807.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  6. A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain
    Authors: E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim
    Nature Communications, 2021-04-06;12(1):2061.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  7. Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D
    Authors: M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim
    Viruses, 2020-07-20;12(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens
    Authors: SH Chang, HJ Kim, CG Park
    Cells, 2020-05-18;9(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production
    Authors: M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl
    Cell. Mol. Immunol., 2019-08-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  11. Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancer
    Authors: MJ Sakiyama, I Espinoza, A Reddy, F de Carlo, A Kumar, AS Levenson, S Bae, X Zhou, PP Claudio, J Lewin, V Manucha, CR Pound, S Vijayakuma, CR Gomez
    Exp. Mol. Pathol., 2019-04-17;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. The homeobox transcription factor MEIS2 is a regulator of cancer cell survival and IMiDs activity in Multiple Myeloma: modulation by Bromodomain and Extra-Terminal (BET) protein inhibitors
    Authors: MP Abruzzese, MT Bilotta, C Fionda, A Zingoni, A Soriani, MT Petrucci, MR Ricciardi, R Molfetta, R Paolini, A Santoni, M Cippitelli
    Cell Death Dis, 2019-04-11;10(4):324.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
    Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
    Sci Rep, 2017-08-17;7(1):8661.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells
    Authors: C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann
    Cell Death Dis, 2017-08-03;8(8):e2973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
    Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
    Oncotarget, 2017-05-09;8(19):31959-31976.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Vigilin Regulates the Expression of the Stress-Induced Ligand MICB by Interacting with Its 5' Untranslated Region
    Authors: O Berhani, D Nachmani, R Yamin, D Schmiedel, Y Bar-On, O Mandelboim
    J. Immunol., 2017-03-29;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. NK-cell receptors NKp46 and NCR1 control human metapneumovirus infection
    Authors: M Diab, A Glasner, B Issacson, Y Bar-On, Y Drori, R Yamin, A Duev-Cohen, O Danziger, R Zamostiano, M Mandelboim, S Jonjic, E Bacharach, O Mandelboim
    Eur. J. Immunol, 2017-03-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study
    Sci Rep, 2016-12-02;6(0):38407.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells
    Sci Rep, 2016-09-27;6(0):32470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. Microchip-Based Single-Cell Imaging Reveals That CD56dimCD57-KIR-NKG2A+ NK Cells Have More Dynamic Migration Associated with Increased Target Cell Conjugation and Probability of Killing Compared to CD56dimCD57-KIR-NKG2A- NK Cells.
    Authors: Forslund E, Sohlberg E, Enqvist M, Olofsson P, Malmberg K, Onfelt B
    J Immunol, 2015-08-28;195(7):3374-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
    Authors: Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
    J Immunol, 2015-06-12;195(2):736-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Coordinated expression of DNAM-1 and LFA-1 in educated NK cells.
    Authors: Enqvist M, Ask E, Forslund E, Carlsten M, Abrahamsen G, Beziat V, Andersson S, Schaffer M, Spurkland A, Bryceson Y, Onfelt B, Malmberg K
    J Immunol, 2015-03-30;194(9):4518-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015-01-29;135(5):1311-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS
  24. Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
    Authors: Kim, Ga Rim, Ha, Ga-Hee, Bae, Jae-Ho, Oh, Sae-Ock, Kim, Sun-Hee, Kang, Chi-Dug
    Oncol Lett, 2015-01-29;9(4):1641-1646.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. RNA-binding proteins regulate the expression of the immune activating ligand MICB.
    Authors: Nachmani, Daphna, Gutschner, Tony, Reches, Adi, Diederichs, Sven, Mandelboim, Ofer
    Nat Commun, 2014-06-13;5(0):4186.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
    Authors: Soriani A, Iannitto M, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi M, Petrucci M, Cippitelli M, Santoni A
    J Immunol, 2014-06-09;193(2):950-60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Flow Cytometry
  27. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
    Authors: Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, Davies J, McSharry B, Weekes M, Antrobus P, Prod'homme V, Blanchet F, Sugrue D, Cuff S, Roberts D, Davison A, Lehner P, Wilkinson G, Tomasec P
    PLoS Pathog, 2014-05-01;10(5):e1004058.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  28. A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
    Authors: Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C
    J Immunol, 2014-04-30;192(11):5332-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  29. MicroRNA editing facilitates immune elimination of HCMV infected cells.
    Authors: Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O
    PLoS Pathog, 2014-02-27;10(2):e1003963.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells.
    Authors: Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
    Cancer Res, 2012-08-21;72(21):5463-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012-03-26;92(7):967-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012-02-22;7(2):e31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  33. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011-02-25;6(2):e16899.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  34. Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
    Authors: Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
    Oncogene, 2011-01-24;30(23):2622-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
    Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG
    J. Exp. Med., 2010-12-20;208(1):13-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009-01-05;113(13):2955-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  37. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
    Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
    Mol. Immunol., 2008-07-26;45(15):3917-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Anti-NKG2D single domain-based antibodies for the modulation of anti-tumor immune response
    Authors: Adeline Raynaud, Klervi Desrumeaux, Laurent Vidard, Elise Termine, Daniel Baty, Patrick Chames et al.
    OncoImmunology
  39. Endothelial Cell Activation and Proliferation Modulate NKG2D Activity by Regulating MICA Expression and Shedding
    Authors: Annabelle Chauveau, Pierre Tonnerre, Angélique Pabois, Pierre-Jean Gavlovsky, Mathais Chatelais, Stéphanie Coupel et al.
    Journal of Innate Immunity

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