Human MIA Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human MIA by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2050) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MIA at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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MIA in Human Melanoma. MIA was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human MIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2050) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Preparation and Storage
Background: MIA
MIA, also named Cartilage-Derived Retinoic Acid-Sensitive Protein (CD-RAP), is a secreted protein that plays an important role in melanoma metastasis. It is highly expressed in malignant melanomas and is associated with tumor progression.
Product Datasheets
Citations for Human MIA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Hypothalamic Rax+ tanycytes contribute to tissue repair and tumorigenesis upon oncogene activation in mice
Authors: W Mu, S Li, J Xu, X Guo, H Wu, Z Chen, L Qiao, G Helfer, F Lu, C Liu, QF Wu
Nature Communications, 2021-04-16;12(1):2288.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Intracellular sortilin expression pattern regulates proNGF-induced naturally occurring cell death during development.
Authors: Nakamura K, Namekata K, Harada C, Harada T
Cell Death Differ., 2007-06-01;14(8):1552-4.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot
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AF2050 was tested along with MAB20501 in a sandwich assay. Although both antibodies worked as either the capture or detection, AF2050 had significantly higher affinity, which allowed quantification at a lower dilution of samples. The version of the assay we ended up using was AF2050 as both the capture and detection.
