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Human Mesothelin PE-conjugated Antibody

Catalog #: FAB32652P
13 Citations 1 Review Datasheet / COA / SDS
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FAB32652P
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Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry.
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Citations (13)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Mesothelin PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mesothelin in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 420411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Gly580
Accession # AAH09272
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Knockout Validated
Mesothelin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Mesothelin knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mesothelin antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin PE‑conjugated Monoclonal Antibody (Catalog # FAB32652P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Mesothelin Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

Mesothelin Specificity is Shown by Flow Cytometry in Knockout Cell Line. Mesothelin knockout HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin PE-conjugated Monoclonal Antibody (Catalog # FAB32652P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). No staining in the Mesothelin knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mesothelin

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

Entrez Gene IDs
10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)
Alternate Names
CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

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Citations for Human Mesothelin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Stromal depletion by TALEN-edited universal hypoimmunogenic FAP-CAR T cells enables infiltration and anti-tumor cytotoxicity of tumor antigen-targeted CAR-T immunotherapy
    Authors: Shipra Das, Julien Valton, Philippe Duchateau, Laurent Poirot
    Frontiers in Immunology
  2. Tumor-targeted non-ablative radiation promotes solid tumor CAR T-cell therapy efficacy
    Authors: Quach, HT;Skovgard, MS;Villena-Vargas, J;Bellis, RY;Chintala, NK;Amador-Molina, A;Bai, Y;Banerjee, S;Saini, J;Xiong, Y;Vista, WR;Byun, AJ;De Biasi, A;Zeltsman, M;Mayor, M;Morello, A;Mittal, V;Gomez, DR;Rimner, A;Jones, DR;Adusumilli, PS;
    Cancer immunology research
    Species: Mouse
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  3. Targeting Immunosuppressive Tumor-Associated Macrophages Using Innate T Cells for Enhanced Antitumor Reactivity
    Authors: YR Li, J Brown, Y Yu, D Lee, K Zhou, ZS Dunn, R Hon, M Wilson, A Kramer, Y Zhu, Y Fang, L Yang
    Cancers, 2022-06-01;14(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. An Ex Vivo 3D Tumor Microenvironment-Mimicry Culture to Study TAM Modulation of Cancer Immunotherapy
    Authors: YR Li, Y Yu, A Kramer, R Hon, M Wilson, J Brown, L Yang
    Cells, 2022-05-08;11(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CRISPR/Cas9-mediated TGFbetaRII disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells in vitro
    Authors: K Alishah, M Birtel, E Masoumi, L Jafarzadeh, HR Mirzaee, J Hadjati, RH Voss, M Diken, S Asad
    Journal of Translational Medicine, 2021-11-27;19(1):482.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Clonal architecture in mesothelioma is prognostic and shapes the tumour microenvironment
    Authors: M Zhang, JL Luo, Q Sun, J Harber, AG Dawson, A Nakas, S Busacca, AJ Sharkey, D Waller, MT Sheaff, C Richards, P Wells-Jord, A Gaba, C Poile, EY Baitei, A Bzura, J Dzialo, M Jama, J Le Quesne, A Bajaj, L Martison, JA Shaw, C Pritchard, T Kamata, N Kuse, L Brannan, P De Philip, H Yang, G Griffiths, G Wilson, C Swanton, F Dudbridge, EJ Hollox, DA Fennell
    Nature Communications, 2021-03-19;12(1):1751.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Genetic and pharmacological targeting of A2a receptor improves function of anti-mesothelin CAR T cells
    Authors: E Masoumi, L Jafarzadeh, HR Mirzaei, K Alishah, K Fallah-Meh, H Rostamian, M Khakpoor-K, R Meshkani, F Noorbakhsh, J Hadjati
    J. Exp. Clin. Cancer Res., 2020-03-10;39(1):49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Recombination of a dual-CAR-modified T lymphocyte to accurately eliminate pancreatic malignancy
    Authors: E Zhang, P Yang, J Gu, H Wu, X Chi, C Liu, Y Wang, J Xue, W Qi, Q Sun, S Zhang, J Hu, H Xu
    J Hematol Oncol, 2018-08-13;11(1):102.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Flow Cytometry
  9. High-throughput screening of rare metabolically active tumor cells in pleural effusion and peripheral blood of lung cancer patients
    Authors: Y Tang, Z Wang, Z Li, J Kim, Y Deng, Y Li, JR Heath, W Wei, S Lu, Q Shi
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition
    J Clin Invest, 2016-07-25;126(8):3130-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  11. An in vivo platform for tumor biomarker assessment.
    Authors: Servais EL, Suzuki K, Colovos C
    PLoS ONE, 2011-10-26;6(10):e26722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. An NK-like CAR T cell transition in CAR T cell dysfunction
    Authors: Charly R. Good, M. Angela Aznar, Shunichiro Kuramitsu, Parisa Samareh, Sangya Agarwal, Greg Donahue et al.
    Cell
  13. Imaging the distribution of an antibody-drug conjugate constituent targeting mesothelin with 89Zr and IRDye 800CW in mice bearing human pancreatic tumor xenografts
    Authors: Eva J. ter Weele, Anton G.T. Terwisscha van Scheltinga, Jos G.W. Kosterink, Linda Pot, Silke R. Vedelaar, Laetitia E. Lamberts et al.
    Oncotarget

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Human Mesothelin PE-conjugated Antibody
By Anonymous on 12/23/2020
Application: Flow Sample Tested: NCI-H226 lung squamous cell carcinoma cells Species: Human
Flow Cytometry Human Mesothelin PE-conjugated Antibody FAB32652P