Human Meprin alpha Subunit/MEP1A Antibody

Catalog #: MAB3220 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3220
MAB3220-SP

All Meprin alpha Subunit/MEP1A Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Meprin alpha Subunit/MEP1A Antibody Summary

Specificity

Detects human Meprin alpha Subunit/MEP1A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MEP1B or recombinant mouse MEP1A is observed.

Source

Monoclonal Mouse IgG_2B Clone # 364312

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Meprin alpha Subunit/MEP1A
Val22-Gln601
Accession # AAA21338

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Meprin alpha Subunit/MEP1A (Catalog # 3220-ZN)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Meprin alpha Subunit/MEP1A (Catalog # 3220-ZN), see our available Western blot detection antibodies

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

HEK293 human embryonic kidney cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Meprin alpha Subunit/MEP1A

Meprins are multimeric proteases composed of alpha and beta subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha subunits with or without beta subunits) and Meprin B (composed of beta subunits only) (3). Although the two subunits share 42% identity in their amino acid (aa) sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). Human Meprin alpha subunit consists of a signal peptide (aa 1 to 21), a pro region (aa 22 to 65), and a mature chain (aa 66 to 746) containing the following domains: catalytic (aa 62 to 263), MAM (aa 264 to 433), MATH (aa 434 to 593), EGF-like (aa 670 to 710), transmembrane (aa 713 to 740), and cytoplasmic (aa 741 to 746).

References

Bond, J.S. and Beynon, R.J. (1995) Protein Sci. 4:1247.
Stocker, W. et al. (1995) Protein Sci. 4:823.
Bertenshaw, G.P., et al. (2001) J. Biol. Chem. 276:13248.
Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Entrez Gene IDs

4224 (Human); 17287 (Mouse); 25684 (Rat)

Alternate Names

bA268F1.1 (meprin A alpha (PABA peptide hydrolase)); EC 3.4.24; EC 3.4.24.18; endopeptidase-2; MEP1A; meprin A subunit alpha; meprin A, alpha (PABA peptide hydrolase); Meprin alpha Subunit; N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha; PABA peptide hydrolase; PPH alpha; PPHA