Human Melanotransferrin/CD228 Antibody

Catalog #: MAB81751 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB81751
MAB81751-SP

Detection of Melanotransferrin/CD228 in A431 Human Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Melanotransferrin/CD228 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Melanotransferrin/CD228 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 893416

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

HEK293 human embryonic kidney cell line transfected with human Melanotransferrin/CD228
Gly20-Gly711
Accession # P08582

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Melanotransferrin/CD228 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Melanotransferrin/CD228 Monoclonal Antibody (Catalog # MAB81751, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melanotransferrin/CD228

Melanotransferrin, also known as MTF, CD228, Melanoma-associated Antigen p97, MAP97 and MFI2, is a 90-97 kDa sialoglycoprotein member of the transferrin family. Unlike other transferrins, which are secreted, MTF is usually found tethered to the cell membrane by a glycosyl phosphatidyl inositol anchor, with only small amounts of soluble protein detected. MTF is highly expressed on melanoma cells, and at lower levels in salivary gland, pancreas, kidney and testis. Like other transferrins, MTF is an iron-binding protein, and may play roles in cellular proliferation, tumorigenesis, metastasis and migration. Full-length human, mouse and rat MTF is 738 amino acids (aa). Over aa 1-711, human MTF shares 85 and 86% aa identity with mouse and rat MTF, respectively.

Entrez Gene IDs

4241 (Human); 30060 (Mouse); 288038 (Rat)

Alternate Names

antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5; CD228 antigen; CD228; FLJ38863; MAP97; MAP97Melanoma-associated antigen p97; Melanotransferrin; membrane-bound transferrin-like protein; MFI2; MGC4856; MTF; MTF1; p97