Human Max Antibody Summary
Asp37-Ser141
Accession # P61244
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Max by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human Max Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for Max was detected at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Detection of Human Max by Western Blot The dimeric inhibitors block Myc:Max but not Max:Max binding to DNA.Gel mobility shift assay showing the effects on Myc:Max DNA complex formation by the dimeric inhibitor E07+N12 (A) and the non-dimerizable control combination E07+C12 (B). The bands that represent protein-DNA complex or naked DNA are shown on the right hand side of each panel. The concentrations indicated are in μM. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25875098), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Max
Max is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. Max is able to form homodimers and heterodimers with other family members (Mad, Mxi1, and Myc). These homodimers and heterodimers compete for a common DNA target site (the E box (CACGTG)). MYC:MAX heterodimers are associated with transcriptional activation and cellular proliferation, whereas, the MAD:MAX heterodimers are associated with transcriptional repression and cellular differentiation. Max has six alternatively spliced transcript variants.
Product Datasheets
Citations for Human Max Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
mRNA interactions with disordered regions control protein activity
Authors: Y Luo, S Pratihar, EH Horste, S Mitschka, ASJS Mey, HM Al-Hashimi, C Mayr
bioRxiv : the preprint server for biology, 2023-02-18;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Reversible linkage of two distinct small molecule inhibitors of Myc generates a dimeric inhibitor with improved potency that is active in myc over-expressing cancer cell lines.
Authors: Wanner, Jutta, Romashko, Darlene, Werner, Douglas, May, Earl W, Peng, Yue, Schulz, Ryan, Foreman, Kenneth, Russo, Suzanne, Arnold, Lee D, Pingle, Maneesh, Bergstrom, Donald E, Barany, Francis, Thomson, Stuart
PLoS ONE, 2015-04-15;10(4):e0121793.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human Max Antibody
There are currently no reviews for this product. Be the first to review Human Max Antibody and earn rewards!
Have you used Human Max Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
