Human Marapsin/Pancreasin Antibody

Catalog #: AF1988 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1988
AF1988-SP

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Human Marapsin/Pancreasin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Marapsin/Pancreasin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross‑reactivity with recombinant mouse Marapsin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Marapsin/Pancreasin
Ala23-Lys290
Accession # Q9BQR3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Marapsin/Pancreasin (Catalog # 1988-SE)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Marapsin/Pancreasin (Catalog # 1988-SE), see our available Western blot detection antibodies

Human Marapsin/Pancreasin Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Human Marapsin/Pancreasin Biotinylated Antibody (Catalog # BAF1988)

Standard: Recombinant Human Marapsin/Pancreasin Protein, CF (Catalog # 1988-SE)

Neutralization

Measured by its ability to neutralize Recombinant Human Marapsin/Pancreasin (0.5 µg/mL, Catalog # 1988-SE) cleavage of the fluorogenic peptide substrate Z-G-R-SBzl (100 µM). The Neutralization Dose (ND₅₀) is typically 2 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Marapsin/Pancreasin

Marapsin, pancreasin, and channel-activating protease 2 (CAP-2) are different names for the same serine protease that is expressed strongly in the pancreas (1, 2). The protein is synthesized with a signal peptide (amino acid residues 1‑22), a pro peptide (residues 23‑34) and a mature chain (residues 35‑290) corresponding to the serine protease domain. The full-length protein was expressed and the secreted protein purified. The N-terminal sequencing results indicate that the purified protein is a disulfide bond-linked dimer formed between the pro peptide and the mature chain. The active enzyme has low activity against peptide substrates tested, but high activity against thioester substrates. The peptidase activity is inhibited by 20 mM benzamidine.

References

Bhagwandin, V.J. et al. (2003) J. Biol. Chem. 278:3363.
Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.

Entrez Gene IDs

83886 (Human); 213171 (Mouse)

Alternate Names

CAP-2; CAPH2; channel-activating protease 2; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; EC 3.4.21.43; Marapsin; MPN; MPNMarapsin; Pancreasin; protease, serine 27; PRSS27; serine protease 27