Human Lymphotoxin beta R/TNFRSF3 Antibody

Catalog #: MAB6291 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6291-100
MAB6291-SP

CXCL8/IL-8 Secretion Induced by Human Lymphotoxin beta R/TNFRSF3 Antibody.

1 Image

All Lymphotoxin beta R/TNFRSF3 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Lymphotoxin beta R/TNFRSF3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Lymphotoxin beta R/TNFRSF3 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 71315

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lymphotoxin beta R/TNFRSF3
Gln31-Met227
Accession # P36941

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Agonist Activity

Measured by its ability to induce CXCL8/IL-8 secretion in the A375 human melanoma cell line. The Neutralization Dose (ND₅₀) is typically 1.5-9 ng/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Agonist Activity

View Larger

CXCL8/IL-8 Secretion Induced by Human Lymphotoxin beta R/TNFRSF3 Antibody. Human Lymphotoxin beta R/TNFRSF3 Monoclonal Antibody (Catalog # MAB6291) activates Lymphotoxin beta Receptor on the A375 human melanoma cell line in a dose-dependent manner which induces CXCL8/IL-8 Secretion, as measured by the Human CXCL8/IL-8 Quantikine ELISA (Catalog # D8000C). The ND₅₀ is typically 1.5-9 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta receptor (LT beta R), also known as TNF RIII and TNF R-related protein (TNF Rrp) is a member of the TNF receptor superfamily, designated TNFRSF3. Human LT beta R cDNA encodes a 435 amino acid (aa) residue type I membrane protein with a putative 30 aa residue signal peptide, a 193 aa residue extracellular domain and a 171 aa residue cytoplasmic domain. The extracellular domain of LT beta R contains four cysteine-rich motifs characteristic of the TNF receptor superfamily. The cytoplasmic region of LT beta R shares little sequence similarity with other TNF receptor family members, suggesting that different signaling mechanisms may be used. LT beta R is expressed in a variety of tissues including visceral and lymphoid tissues. LT beta R is also expressed by cell lines of monocytic, epithelial, and fibroblastic origins but not by T and B lymphocytes. Human and mouse LT beta R share 76% aa sequence homology. The TNF family ligands that have been shown to bind and activate LT beta R include LIGHT (also a ligand for HVEM) and the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. Depending on the cell type, activation of LT beta R has been shown to induce NF kappa B activation, chemokine production, growth arrest, and apoptosis. In vivo, LT beta R has been shown to play a critical role in controlling cellular immune functions and lymphoid organogenesis.

References

Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
Rennert, P.D. et al. (1998) Immunity 9:71.
Degli-Esposti, M.A. et al. (1997) J. Immunol 158:1756.
Mackay, F. et al. (1996) J. Biol. Chem. 271:8618.
Crowe, P.D. et al. (1994) Science 264:707.

Long Name

Lymphotoxin beta Receptor

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Alternate Names

CD18; D12S370; ltbetar; LT-BETA-R; LTBR; lymphotoxin B receptor; Lymphotoxin beta R; lymphotoxin beta receptor (TNFR superfamily, member 3); Lymphotoxin-beta receptor; LymphotoxinbR; TNF RIII; TNF Rrp; TNFCRTNF-RIII; TNFR superfamily, member 3; TNFR2-RP; TNFR3; TNF-R-III; TNFR-RP; TNFRSF3; TNFRSF3TNFR-III; Tumor necrosis factor C receptor; Tumor necrosis factor receptor 2-related protein; tumor necrosis factor receptor superfamily member 3; tumor necrosis factor receptor superfamily, member 3; Tumor necrosis factor receptor type III