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Human LRRC52 Antibody

Catalog #: MAB10260 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB10260-100
MAB10260-SP
Detection of LRRC52 in HEK293 Human Cell Line Transfected with Human LRRC52 and eGFP by Flow Cytometry
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Human LRRC52 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LCCC52 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1037552
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell HEK293-derived human LRRC52
Ser24-Asp244
Accession # Q8N7C0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
HEK293 Human Cell Line Transfected with Human LRRC52 and eGFP

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of LRRC52 in HEK293 Human Cell Line Transfected with Human LRRC52 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with (A) human LRRC52 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human LRRC52 Monoclonal Antibody (Catalog # MAB10260) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LRRC52

LRRC52 (Leucine-rich repeat-containing protein 52) belongs to an extracellular leucine-rich-repeat–only (Elron) cluster, which includes five other LRRC proteins: LRRC26, LRRC38, LRRC55, LRTM1 and LRTM2 (1). Elron cluster members are type I transmembrane proteins with an extracellular domain (ECD) containing several LRR motifs and a cytoplasmic C-terminal tail with a short stretch of acidic residues (1). The mature ECD of LRRC52 is 221 amino acids (aa), containing 5 LRR motifs, and shares 76% and 78% identity with mouse and rat LRRC52, respectively. LRRC-52 is dominantly expressed in testis and skeletal muscle and is also detected in several other tissues including placenta, kidney, lung, and some glands (2, 3). LRRC52 has been identified as a testes specific SLO3 K+ channel accessory protein in mouse (2). LRRC52 is proposed to be essential for alkalization dependent activation of the KSper current at physiological membrane potentials and pH (2). Activation of the SLO3 channel is critical in the fertilization process and loss of function results in infertility (2, 4, 5). A second Elron cluster protein, LRRC26, was recently identified as a regulatory subunit of SLO1 BK-type K+ channels, suggesting a general function for the entire family (6). Based on function, LRRC52 might be a therapeutic target for male contraception (7).

References
  1. Dolan, J. et al. (2007) BMC Genomics 8:320.
  2. Yang, C. et al. (2011) PNAS 108:19419.
  3. Leonetti, M. et al. (2012) PNAS. 109:19274.
  4. Navarro, B. et al. (2007) PNAS 104:7688.
  5. Sanit, C. et al. (2010) FEBS Lett. 584:1041.
  6. Yan, J. and Aldrich, RW. (2010) Nature 466:513.
  7. Zeng, XH. et al. (2015) PNAS 112:2599.
Long Name
Leucine-rich Repeat Containing 52
Entrez Gene IDs
440699 (Human); 240899 (Mouse); 289199 (Rat)
Alternate Names
BK Channel Auxiliary Gamma Subunit LRRC52; FLJ25811; leucine rich repeat containing 52; leucine-rich repeat-containing protein 52; LRRC52

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