Human LMP7/PSMB8 Antibody

Catalog #: MAB7710 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7710
MAB7710-SP

Detection of Human LMP7/PSMB8 by Western Blot.

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All LMP7/PSMB8 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LMP7/PSMB8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LMP7/PSMB8 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LMPX/PSMB5 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 832913

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human LMP7/PSMB8
Thr73-Gln276
Accession # P28062

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human LMP7/PSMB8 by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human LMP7/ PSMB8 Monoclonal Antibody (Catalog # MAB7710) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMP7/PSMB8 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LMP7/PSMB8

PSMB8 (Proteasome Subunit beta type-8; Also beta 5i, RING10/Y2 and LMP7) is a 23-24 kDa member of the peptidase T1B family of molecules. It is expressed both constitutively and inducibly by IFN-gamma in a wide variety of cells, including immature dendritic cells, preadipocytes, CD4⁺ T cells and monocytes. LMP7 is a subunit of the 700 kDa, 20S proteasome catalytic complex, a dynamic intracellular structure that participates in ATP-dependent proteolytic activity. LMP7 qualifies as a beta -type, i (immuno)-type proteasome, meaning it both plays a chymotrypsin-like role in the turnover of proteins and is found in cytokine-responsive cells. The peptides generated through LMP7 activity are presented as antigens by MHC-I molecules. LMP7 activity is dependent upon the removal of the LMP7 precursor prosequence, an action that exposes a critical internal Thr residue. Human LMP7 is synthesized as a 28-29 kDa, 276 amino acid (aa) proprecursor. It contains a 72 aa autocleavable propeptide plus a 204 aa mature region. There is one alternative splice form that shows a 45 aa substitution for aa 1-49. This isoform does not appear to participate in formation of a proteosome. Over aa 73-276, human LMP7 shares 92% aa sequence identity with mouse LMP7.

Long Name

Large Multifunctional Protease 7

Entrez Gene IDs

5696 (Human)

Alternate Names

ALDD; beta5i; D6S216E; EC 3.4.25.1; JMP; LMP7; LMP7MGC1491; Low molecular mass protein 7; low molecular weight protein 7; Macropain subunit C13; Multicatalytic endopeptidase complex subunit C13; NKJO; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalpeptidase 7); proteasome catalytic subunit 3i; Proteasome Component C13; proteasome subunit beta 5i; proteasome subunit beta type-8; Proteasome subunit beta-5i; Proteasome Subunit Y2; proteasome-related gene 7; PSMB5i; PSMB5iD6S216; PSMB8; Really interesting new gene 10 protein; RING10; RING10proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalprotease 7); Y2