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Human LILRB5/CD85c/LIR-8 Antibody

Catalog #: AF3065
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3065
AF3065-SP
Detection of LILRB5/CD85c/LIR-8 in Human peripheral blood monocytes by Flow Cytometry.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LILRB5/CD85c/LIR-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human LILRB5/CD85c/LIR-8 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LILRA4/ILT7 is observed and less than 15% cross-reactivity with rhILT3 and rhILT5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LILRB5/CD85c/LIR-8
Arg18-His456
Accession # O75023
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1-0.5 µg/mL
Recombinant Human LILRB5/CD85c/LIR-8, human bone marrow
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LILRB5/CD85c/LIR-8 antibody in Human peripheral blood monocytes antibody by Flow Cytometry. View Larger

Detection of LILRB5/CD85c/LIR-8 in Human peripheral blood monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human LILRB5/CD85c/LIR-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3065, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Western Blot View Larger

Detection of Human LILRB5/CD85c/LIR-8 by Western Blot. Western blot shows lysates of Human bone marrow. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LILRB5/CD85c/LIR-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for LILRB5/CD85c/LIR-8 at approximately 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LILRB5/CD85c/LIR-8

Human LIR-8 (also known as CD85c and LILRB5) is a 587 amino acid transmembrane protein of the family of Leukocyte Immunoglobin-like Receptors. LIR-8 has a long N-terminus extracellular domain with four C2-type lg-like domains, and a short cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) typically involved in modulation of cellular responses. Human LIR-8 is expressed in natural killer (NK) cells. There are at least two other known isoforms, one with 53 aa longer cytoplasmic domain, the other with a 100 aa deletion affecting one of the lg-like extracellular domains.

Long Name
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM And ITIM Domains), Member 5
Entrez Gene IDs
10990 (Human); 102129427 (Cynomolgus Monkey)
Alternate Names
CD85 antigen-like family member C; CD85c antigen; CD85c; Leukocyte immunoglobulin-like receptor 8; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains); LILRB5; LIR8; LIR-8; LIR8CD85C; LIR-8leukocyte immunoglobulin-like receptor subfamily B member 5; member 5

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Citations for Human LILRB5/CD85c/LIR-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Coexpression of CD163 and CD141 identifies human circulating IL-10-producing dendritic cells (DC-10)
    Authors: M Comi, D Avancini, F Santoni de, M Villa, MJ Uyeda, M Floris, D Tomasoni, A Bulfone, MG Roncarolo, S Gregori
    Cell. Mol. Immunol., 2019-03-06;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation
    Nat Commun, 2016-05-05;7(0):11473.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains.
    Authors: Zhang Z, Hatano H, Shaw J, Olde Nordkamp M, Jiang G, Li D, Kollnberger S
    PLoS ONE, 2015-06-22;10(6):e0129063.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Inhibitory receptors bind ANGPTLs and support blood stem cells and leukaemia development.
    Authors: Zheng J, Umikawa M, Cui C, Li J, Chen X, Zhang C, Hyunh H, Kang X, Silvany R, Wan X, Ye J, Canto AP, Chen SH, Wang HY, Ward ES, Zhang CC
    Nature, 2012-05-30;485(7400):656-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human LILRB5/CD85c/LIR-8 Antibody
By Anonymous on 11/29/2022
Application: ELISA Sample Tested: Serum Species: Human
ELISA Human LILRB5/CD85c/LIR-8 Antibody AF3065