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Human Leptin/OB Antibody

Catalog #: AF398
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF398
AF398-SP
Detection of Recombinant Human, Mouse, and Rat Leptin/OB by Western Blot.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Leptin/OB Antibody Summary

Species Reactivity
Human
Specificity
Detects human Leptin/OB in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant mouse Leptin/OB is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Leptin/OB
Val22-Cys167
Accession # Q6NT58
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Leptin/OB-induced proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R. The Neutralization Dose (ND50) is typically 0.5-3.0 µg/mL in the presence of 7.5 ng/mL Recombinant Human Leptin/OB.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human, Mouse, and Rat Leptin/OB antibody by Western Blot. View Larger

Detection of Recombinant Human, Mouse, and Rat Leptin/OB by Western Blot. Western blot shows 100 ng of Recombinant Human Leptin/OB (Catalog # 398-LP), Recombinant Mouse Leptin/OB (Catalog # 498-OB) and Recombinant Rat Leptin/OB (Catalog # 598-LP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Leptin/OB at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry Leptin/OB antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

Leptin/OB in Human Stomach. Leptin/OB was detected in immersion fixed paraffin-embedded sections of human stomach using Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. View Larger

Cell Proliferation Induced by Leptin/OB and Neutralization by Human Leptin/OB Antibody. Recombinant Human Leptin/OB (Catalog # 398-LP) stimulates proliferation in the BaF3 mouse pro‑B cell line transfected with human Leptin R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Leptin/OB (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Leptin/OB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF398). The ND50 is typically 0.5-3.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Leptin/OB

Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.

References
  1. Considine, R. and J. Caro (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al. (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.
Entrez Gene IDs
3952 (Human); 16846 (Mouse); 25608 (Rat)
Alternate Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS

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Citations for Human Leptin/OB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Reversion of breast epithelial polarity alterations caused by obesity
    Authors: Julia Holmes, Mohamed Gaber, Mónica Z. Jenks, Adam Wilson, Tucker Loy, Cassandra Lepetit et al.
    npj Breast Cancer
  2. Leptin, adiponectin, and their ratio as markers of insulin resistance and cardiometabolic risk in childhood obesity
    Authors: Christine Frithioff‐Bøjsøe, Morten A.V. Lund, Ulrik Lausten‐Thomsen, Paula L. Hedley, Oluf Pedersen, Michael Christiansen et al.
    Pediatric Diabetes
  3. UV-induced inhibition of adipokine production in subcutaneous fat aggravates dermal matrix degradation in human skin
    Sci Rep, 2016-05-10;6(0):25616.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Increased leptin expression in endometriosis cells is associated with endometrial stromal cell proliferation and leptin gene up-regulation.
    Authors: Wu MH, Chuang PC, Chen HM
    Mol. Hum. Reprod., 2002-05-01;8(5):456-64.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  5. Metastasis-associated MCL1 and P16 copy number alterations dictate resistance to vemurafenib in a BRAFV600E patient-derived papillary thyroid carcinoma preclinical model
    Authors: Mark Duquette, Peter M. Sadow, Amjad Husain, Jennifer N. Sims, Zeus A. Antonello, Andrew H. Fischer et al.
    Oncotarget
  6. Cellular Expression of Cyclooxygenase, Aromatase, Adipokines, Inflammation and Cell Proliferation Markers in Breast Cancer Specimen
    Authors: Samar Basu, Kristell Combe, Fabrice Kwiatkowski, Florence Caldefie-Chézet, Frédérique Penault-Llorca, Yves-Jean Bignon et al.
    PLOS ONE

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