Human Layilin Biotinylated Antibody

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BAF2637
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Human Layilin Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Layilin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant mouse Layilin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Layilin
Ala22-Glu220
Accession # NP_849156
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Layilin (Catalog # 2536-LA)

Human Layilin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Layilin Antibody (Catalog # MAB26371)

Standard: Recombinant Human Layilin Protein (Catalog # 2536-LA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Layilin

Layilin (named after the L-A-Y-I-L-I six amino acid motif in the molecule’s transmembrane segment) is a 55 kDa member of the animal C-type lectin family. The human Layilin cDNA encodes a 374 amino acid (aa) type I transmembrane protein that, based on the orthologous molecule in hamster, contains a 19 aa signal sequence, a 201 aa extracellular region, a 31 aa transmembrane segment, and a 123 aa cytoplasmic tail (1, 2). The extracellular domain has a 130 aa carbohydrate recognition domain that contains only one potential Ca++-binding site. The intracellular region contains three types of LH (Layilin homology) repeats. There are three 16‑18 aa LH1 repeats, 3 five aa (E-G-S-F/W-V) LH2 repeats, and 2 four aa (N-D/E-I-Y) LH3 repeats. Adjacent LH2-LH3 tandem arrays provide docking sites for talin, a cytoskeleton-membrane adaptor protein (3). The human Layilin extracellular region is 85% aa identical to the hamster Layilin extracellular region, and 82% aa identical to the extracellular region of a similar molecule identified in mouse. Layilin is known to be a receptor for hyaluronan (HA), a negatively charged linear high molecular weight polysaccharide composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine (1, 4, 5). HA is found in the extracellular matrix of most animal tissues and in body fluids. Thus, it may be that Layilin modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. Layilin binds specifically to HA but not to other glycosaminoglycans (4).

References
  1. Strausberg, R. (2002) GenBank Accession # AAH25407.
  2. Borowsky, M.L. and R.O. Hynes (1998) J. Cell Biol. 143:429.
  3. Rees, D.J.G. et al. (1990) Nature 347:685.
  4. Bono, P. et al. (2001) Mol. Biol. Cell 12:891.
  5. Goa, K.L. and P. Benfield (1994) Drugs 47:536.
Entrez Gene IDs
143903 (Human); 244864 (Mouse)
Alternate Names
FLJ30977; FLJ31092; Layilin; LAYN; LYAN

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