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Human L-Selectin/CD62L Antibody

Catalog #: BBA24
9 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BBA24
Detection of L‑Selectin/CD62L in PBMC lymphocytes by Flow Cytometry
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Citations (9)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human L-Selectin/CD62L Antibody Summary

Species Reactivity
Human
Specificity
Binds to CHO cells expressing the human L‑Selectin/CD62L protein. It does not recognize CHO cells expressing either the human E-Selectin or human P-Selectin.
Source
Monoclonal Mouse IgG1 Clone # 4G8
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant human L‑Selectin/CD62L
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human whole blood lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Human L-Selectin/CD62L Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human L-Selectin/CD62L Biotinylated Antibody (Catalog # BAF728)

Standard: Recombinant Human L-Selectin/CD62L Protein, CF (Catalog # ADP2)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of L‑Selectin/CD62L in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human L‑Selectin/CD62L Monoclonal Antibody (Catalog # BBA24) or (B) isotype control antibody (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: L-Selectin/CD62L

L-Selectin (LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L), is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. It plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites.

Entrez Gene IDs
6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)
Alternate Names
CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1

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Citations for Human L-Selectin/CD62L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Augmentation of Human Monocyte Responses to Lipopolysaccharide by the Protein S and Mer/Tyro3 Receptor Tyrosine Kinase Axis
    Authors: ND Barth, JA Marwick, MJ Heeb, AJ Gale, AG Rossi, I Dransfield
    J. Immunol., 2018-09-24;0(0):.
  2. NFKB1 regulates human NK cell maturation and effector functions
    Authors: Vassilios Lougaris
    Clin. Immunol, 2016-12-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Regulatory T cells inhibit CD34+ cell differentiation into NK cells by blocking their proliferation.
    Authors: Isabela Pedroza-Pa, Divya Shah, Anna Domogala, Martha Luevano, Michael Blundell, Nicola Jackson, Adrian Thrasher, Alejandro Madrigal, Aurore Saudemont
    Scientific Reports, 2016-02-26;0(0):2045-2322.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Facile supermolecular aptamer inhibitors of L-selectin.
    Authors: Chang, Elizabet, Eckert, Mark A, Ali, M Monsur, Riazifar, Hamidrez, Pone, Egest J, Liu, Linan, Zhao, Weian
    PLoS ONE, 2015-03-31;10(3):e0123034.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  5. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function.
    Authors: Zhang Y, Patel B, Dey A, Ghorani E, Rai L, Elham M, Castleton AZ, Fielding AK
    J. Immunol., 2011-12-16;188(3):1002-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004-06-01;112(2):274-89.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry
  7. The soluble form of IL-1 receptor accessory protein enhances the ability of soluble type II IL-1 receptor to inhibit IL-1 action.
    Authors: Smith DE, Hanna R, Della Friend, Moore H, Chen H, Farese AM, MacVittie TJ, Virca GD, Sims JE
    Immunity, 2003-01-01;18(1):87-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. A novel and accurate microfluidic assay of CD62L in bladder cancer serum samples
    Authors: Gayatri S. Phadke, Jennifer E. Satterwhite-Warden, Dharamainder Choudhary, John A. Taylor, James F. Rusling
    The Analyst
  9. Quantifying the Biomechanics of Conception: L-Selectin-Mediated Blastocyst Implantation Mechanics with Engineered “Trophospheres”
    Authors: Robert W. Yucha, Monika Jost, Dianne Rothstein, Noreen Robertson, Michele S. Marcolongo
    Tissue Engineering Part A

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