Home / Kynureninase / Human Kynureninase Antibody

Human Kynureninase Antibody

Catalog #: AF4887
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4887
AF4887-SP
Detection of Human Kynureninase by Western Blot.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kynureninase Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kynureninase in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Kynureninase
Met1-Asn465
Accession # Q16719
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kynureninase (Catalog # 4887-KH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Kynureninase antibody by Western Blot. View Larger

Detection of Human Kynureninase by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Kynureninase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4887) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynureninase

Kynureninase is a pyridoxal-5 (-phosphate-dependent enzyme that catalyzes the hydrolytic cleavage of the amino acids L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3-hydroxyanthranilic acid and alanine (1). The enzyme is a member of the “kynurenine pathway” enzymes, through which the majority of dietary tryptophan is degraded in the liver, and is involved in the de novo biosynthesis of NAD+ (2, 3). Kynurenine pathway genes are expressed in immune system cells such as macrophages and microglia. During inflammatory responses, the kynurenine pathway in these cells produces quinolinic acid (QA) and not NAD+. QA excites neurons via the activation of NMDA (N-methyl-D-aspartate) receptors resulting in neuronal damage. The tissue-damaging process has been demonstrated in AIDS-related dementia complex, Alzheimer’s, stroke, epilepsy, and Huntington’s disease. Because Kynureninase is one of the key enzymes of QA production, its inhibitors may be useful for the treatment of neurological disorders. The recombinant Kynureninase has been shown to possess specificity for 3-hydroxykynurenine over kynurenine (4, 5).

References
  1. Lima, S. et al. (2007) Biochemistry 46:2735.
  2. Botting, N. P. (1995) Chem. Soc. Rev. 24:401.
  3. Stone, T. W. (2000) Trends in Pharm. Sci. 21:149.
  4. Walsh, H. et al. (2002) Eur. J. Chem. 269:2069.
  5. Toma, S. et al. (1997) FEBS Lett. 408:5.
Entrez Gene IDs
8942 (Human); 70789 (Mouse); 116682 (Rat)
Alternate Names
EC 3.7.1.3; KYNU; kynureninase (L-kynurenine hydrolase); Kynureninase; L-kynurenine hydrolase

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Kynureninase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Protective Role of Kynurenine 3-Monooxygenase in Allograft Rejection and Tubular Injury in Kidney Transplantation
    Authors: Lassiter R, Merchen Td, Fang X, Wang Y
    Frontiers in immunology
  2. HIV-1 Envelope Mimicry of Host Enzyme Kynureninase Does Not Disrupt Tryptophan Metabolism
    Authors: Barton F Haynes
    J. Immunol., 2016-11-14;0(0):.
    Applications: Neutralization
  3. Identification of autoantigens recognized by the 2F5 and 4E10 broadly neutralizing HIV-1 antibodies.
    Authors: Yang, Guang, Holl, T Matt, Liu, Yang, Li, Yi, Lu, Xiaozhi, Nicely, Nathan I, Kepler, Thomas B, Alam, S Munir, Liao, Hua-Xin, Cain, Derek W, Spicer, Leonard, VandeBerg, John L, Haynes, Barton F, Kelsoe, Garnett
    J Exp Med, 2013-01-28;210(2):241-56.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Kynureninase Antibody

There are currently no reviews for this product. Be the first to review Human Kynureninase Antibody and earn rewards!

Have you used Human Kynureninase Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review