Human Kynureninase Antibody

Catalog #: MAB4887R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB4887R-100
MAB4887R-SP

Detection of Human Kynureninase by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kynureninase Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kynureninase in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG₁ Clone # 589731R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Kynureninase
Met1-Asn465
Accession # Q16719

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Kynureninase by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kynureninase Monoclonal Antibody (Catalog # MAB4887R) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Kynureninase at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynureninase

Kynureninase (KYNU) catalyzes the hydrolytic cleavage of the amino acids L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3‑hydroxyanthranilic acid and alanine. KYNU and other “kynurenine pathway” enzymes degrade dietary tryptophan in the liver and are involved in the de novo biosynthesis of NAD⁺. KYNU and other pathway proteins in immune system cells, such as macrophages and microglia, catalyze inflammatory quinolinic acid (QA) production, which may cause neuronal damage in AIDS-related dementia complex, Alzheimer’s, stroke, epilepsy, and Huntington’s disease. Human KYNU shares 83% and 85% amino acid identity with mouse and human KYNU, respectively.

Entrez Gene IDs

8942 (Human); 70789 (Mouse); 116682 (Rat)

Alternate Names

EC 3.7.1.3; KYNU; kynureninase (L-kynurenine hydrolase); Kynureninase; L-kynurenine hydrolase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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