Human KIR3DL3/CD158z Antibody

Catalog #: MAB8919
1 Citation Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8919
MAB8919-SP

Detection of KIR3DL3/CD158z in BaF3 Mouse Cell Line Transfected with Human KIRDL3/CD158z by Flow Cytometry.

1 Image

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human KIR3DL3/CD158z Antibody Summary

Species Reactivity

Human

Specificity

Detects human KIR3DL3/CD158z in direct ELISAs.

Source

Monoclonal Rabbit IgG Clone # 1136B

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of KIR3DL3/CD158z in BaF3 Mouse Cell Line Transfected with Human KIRDL3/CD158z by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human KIRDL3/CD158z was stained with Rabbit Anti-Human KIR3DL3/CD158z Monoclonal Antibody (Catalog # MAB8919, filled histogram) or Rabbit Anti-Human KIR2DS5/CD158g Monoclonal Antibody (Catalog # MAB8920, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: KIR3DL3/CD158z

KIR3DL3 is a member of the killer-immunoglobulin-like receptors (KIR), a functionally diverse family of transmembrane receptors expressed primarily on NK cells. KIR3DL3 is a “framework” KIR gene, and is present in all individuals, in contrast to non-framework KIRs, whose gene content varies by KIR haplotype. KIR3DL3 is expressed at low or undetectable levels in peripheral blood NK cells, but expression can be induced in NK cell lines by demethylation agents, indicating that promoter DNA methylation inhibits KIR3DL3 transcription. Despite the lack of evidence for surface protein expression, KIR3DL3 mRNA expression has been detected in both decidual and peripheral blood NK cells in normal, disease-free conditions.

Long Name

Killer Cell Immunoglobulin-like Receptor, Three Domain Long Cytoplasmic Tail, 3

Entrez Gene IDs

115653 (Human)

Alternate Names

CD158z; CD158Z;Killer cell immunoglobulin-like receptor 3DL3;KIR2DL5B;KIR3DL7;KIR44;KIRC1; KIR3DL3; KIR3DL7; KIR44; KIRC1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human KIR3DL3/CD158z Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1