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Human Kallikrein 8/Neuropsin Antibody

Catalog #: MAB17191
1 Citation Datasheet / COA / SDS
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MAB17191
MAB17191-SP
Product Details
Citations (1)
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Human Kallikrein 8/Neuropsin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 8/Neuropsin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) UPA‑1, rhKallikrein 1, 2, 3, 4, 5, 7, 9, 10, 11, 12, 13, 14, 15, rhKallikrein B1, rhHGFA, rhFactor VII, rhFactor X, rhFactor XI, rhProc, or rhThrombin is observed.
Source
Monoclonal Rat IgG1 Clone # 369910
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 8/Neuropsin
Gln29-Gly260 (predicted)
Accession # O60259
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 8/Neuropsin (Catalog # 2025-SE), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Kallikrein 8/Neuropsin (0.5 µg/mL, Catalog # 2025-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-Amc (0.1 mM, Catalog # ES011). The Neutralization Dose (ND50) is typically 0.7 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 8/Neuropsin

Kallikrein 8 (KLK8), also known as neuropsin or ovasin, is a member of the human tissue kallikrein family (1). Two alternatively spliced forms exist, resulting in 260 (isoform 1) and 305 (isoform 2) amino acid (aa) sequences, respectively (2). Isoform 1 consists of a signal peptide (residues 1 to 28), a short pro peptide (aa 29‑32) and the mature chain (aa 33-260). Isoform 2 is identical to isoform 1, except that a 45 aa segment is inserted in isoform 2 between aa 23 and 24 in isoform 1. Isoform 1 is predominantly expressed in pancreas whereas isoform 2 is preferentially expressed in adult brain and hippocampus, although both forms are expressed in fetal brain and placenta in comparable levels. The brain function of KLK8 seems evident in neuropsin knockout mice that showed abnormalities of synapses and neurons and predisposation to global seizure activity (3, 4). KLK8 is a novel marker for ovarian and cervical cancer carcinomas (5, 6). Recombinant human KLK8, after being activated by lysyl endopeptidase, can cleave fibronectin and several small peptide substrates (7, 8).

References
  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Mitsui, S. et al. (1999) Eur. J. Biochem. 260:627.
  3. Hirata, A. et al. (2001) Mol. Cell. Neurosci. 17:600.
  4. Davies, B. et al. (2001) J. Neurosci. 21:6993.
  5. Kishi, T. et al. (2003) Cancer Res. 63:2771.
  6. Cane, S. et al. (2004) Am. J. Obstet. Gynecol. 190:60.
  7. Oka, T. et al. (2002) J. Biol. Chem. 277:14724.
  8. Shimizu, C. et al. (1998) J. Biol. Chem. 273:11189.
Entrez Gene IDs
11202 (Human)
Alternate Names
EC 3.4.21; EC 3.4.21.118; hK8; HNP; kallikrein 8 (neuropsin/ovasin); Kallikrein 8; kallikrein-related peptidase 8; KLK8 protein type 1; KLK8 protein type 2; KLK8; neuropsin type 1; neuropsin type 2; Neuropsin; NP; NRPN; Ovasin; PRSS19; PRSS19kallikrein-8; Serine protease 19; Serine protease TADG-14; TADG14neuropsin; Tumor-associated differentially expressed gene 14 protein

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Citation for Human Kallikrein 8/Neuropsin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Inhibition of transcription factor specificity protein 1 alters the gene expression profile of keratinocytes leading to upregulation of kallikrein-related peptidases and thymic stromal lymphopoietin.
    Authors: Bin L, Kim BE, Hall CF
    J. Invest. Dermatol., 2011-07-14;131(11):2213-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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