Human Kallikrein 15 Biotinylated Antibody

Catalog #: BAF2540 Datasheet / COA / SDS

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BAF2540

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Kallikrein 15 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Kallikrein 15 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) KLK2, rhKLK8, and rhKLK11 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kallikrein 15 isoform 1
Asp17-Asn255
Accession # AAI44047

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Kallikrein 15

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 15

Kallikrein 15, encoded by the KLK15 gene, is a member of the tissue Kallikrein family of Serine proteases. It is a secreted protein found in prostate, colon, thyroid tissues, breast milk and seminal plasma. Whether it has a trypsin-like activity or not remains controversial. Several splice forms exist. The 250 aa rhKLK15 has a 10‑His tag at the C-terminus. It predicts a molecular mass of 28 kDa and migrates as a 39 kDa band in SDS-PAGE under reducing conditions. The amino acid sequence of human KLK15 is 99%, 77% and 77% identical to that of chimpanzee, canine and mouse, respectively.

Entrez Gene IDs

55554 (Human)

Alternate Names

ACO protease; ACO; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; HSRNASPH; Kallikrein 15; kallikrein-15; kallikrein-like serine protease; kallikrein-related peptidase 15; KLK15; Prostinogen