Human Kallikrein 15 Antibody

Catalog # Availability Size / Price Qty
MAB2540
MAB2540-SP
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Human Kallikrein 15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 15 in Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Factor X is observed and no cross-reactivity with recombinant mouse HGFA, rhKallikrein 3, 5, 6, 8, 10, 11, rhThrombin, or rhuPA is observed.
Source
Monoclonal Mouse IgG2A Clone # 252819
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 15 isoform 1
Leu23-Asn256 (predicted)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Kallikrein 15 under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Kallikrein 15, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 15

Kallikrein 15 is a serine protease of the tissue Kallikrein family that is upregulated in prostate cancer.

Entrez Gene IDs
55554 (Human)
Alternate Names
ACO protease; ACO; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; HSRNASPH; Kallikrein 15; kallikrein-15; kallikrein-like serine protease; kallikrein-related peptidase 15; KLK15; Prostinogen

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