Human ISG15/UCRP APC-conjugated Antibody Summary
Accession # P05161
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of ISG15/UCRP in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (light orange filled histogram) or treated with 500 U/mL Recombinant Human IFN-beta 1a (Mammalian) (Catalog # 11415-1) for 16 hours (dark orange filled histogram) was stained with Rat Anti-Human ISG15/UCRP APC-conjugated Monoclonal Antibody (Catalog # IC8044A) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ISG15/UCRP
ISG15 (interferon-induced 15 kDa protein; also IP17) is a 15 kDa ubiquitin family protein that is expressed by a wide variety of cell types. ISG15 is induced by type-I IFN, and like ubiquitin, becomes conjugated to intracellular proteins. Unlike ubiquitin, it does not target proteins for degradation. Instead, it is part of the antivirus response. Human proISG15 is 165 amino acids (aa) in length. There are two ubiquitin-like domains (aa 2-78 and 79-157) and a propeptide (aa 158-165) that is cleaved intracellularly. Select blood mononuclear cells secrete mature ISG15, which is chemotactic for neutrophils. Mature human ISG15 is 66% aa identical to mouse ISG15.
Product Datasheets
Citation for Human ISG15/UCRP APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Human-Specific Adaptations in Vpu Conferring Anti-tetherin Activity Are Critical for Efficient Early HIV-1 Replication In�Vivo
Authors: E Yamada, S Nakaoka, L Klein, E Reith, S Langer, K Hopfensper, S Iwami, G Schreiber, F Kirchhoff, Y Koyanagi, D Sauter, K Sato
Cell Host Microbe, 2018-01-10;23(1):110-120.e7.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human ISG15/UCRP APC-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human ISG15/UCRP APC-conjugated Antibody and earn rewards!
Have you used Human ISG15/UCRP APC-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
