Human IQGAP2 Antibody

Catalog #: AF5068 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5068
AF5068-SP

Detection of Human IQGAP2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IQGAP2 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human IQGAP2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IQGAP2
Asn444-Ser633
Accession # Q13576

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IQGAP2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh-7 human hepatoma cell line, Ramos human Burkitt's lymphoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human IQGAP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5068) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IQGAP2 at approximately 185 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IQGAP2

IQGAP2 (IQ motif with Ras GAP-related domain 2) is a 180‑190 kDa member of the IQGAP family of proteins. It has limited expression (hepatocytes, platelets, parietal cells), binds to GTP-bound Cdc42, blocking GTP hydrolysis, and participates in actin cross-linking. Human IQGAP2 is 1575 amino acids (aa) in length. It contains one actin-binding CH domain (aa 41‑156), a Pro-binding WWP region (aa 594‑627), three consecutive calmodulin-binding IQ motifs (aa 690‑779), and a noncatalytic Ras-GAP domain (aa 917‑1150). There are multiple IQGAP2 splice variants. One shows a seven aa substitution for aa 1‑454 coupled to a deletion of aa 642‑698, while a second shows a 67 aa substitution for aa 303 and 304. IQGAP-2 is 59% aa identical to IQGAP1.

Long Name

IQ Motif Containing GTPase Activating Protein 2

Entrez Gene IDs

10788 (Human); 544963 (Mouse)

Alternate Names

IQ motif containing GTPase activating protein 2; IQGAP2; ras GTPase-activating-like protein IQGAP2