Human Intelectin-1/Omentin Antibody

Catalog #: MAB4254 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4254-100
MAB4254-SP

Human Intelectin‑1/Omentin ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Intelectin-1/Omentin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Intelectin-1/Omentin in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 450520

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Intelectin-1/Omentin
Thr19-Ser298
Accession # Q8WWA0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Intelectin‑1/Omentin Monoclonal Antibody Catalog # MAB42541.

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Intelectin‑1/Omentin ELISA Standard Curve. Recombinant Human Intelectin-1/Omentin protein was serially diluted 2-fold and captured by Mouse Anti-Human Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB4254) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Intelectin-1/Omentin Monoclonal Antibody (Catalog # MAB42541) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Intelectin-1/Omentin

Intelectin-1 (Intestinal Lectin; also ITLN-1 and Omentin) is a 40 kDa Ca-dependent galactofuranose-binding lectin that is not a C-type lectin. It is expressed on multiple cell types and appears to participate in insulin signaling and microbe recognition. The human ITLN-1 preproprecursor is 313 amino acids (aa) in length. It contains an 18 aa signal sequence, a 280 aa mature segment (aa 19-298), and a 15 aa C-terminal proregion that is cleaved to generate a GPI-linkage. One fibrinogen-related domain exists in the mature molecule (aa 36-209). Human ITLN-1 exists as a disulfide-linked glycosylated homotrimer. Mouse ITLN-1 is unglycosylated and a monomer. Mature human ITLN-1 is 82% aa identical to mouse ITLN-1. Human ITLN-1 shows one alternate start site at Met72, and a splice form that contains a 32 aa substitution for aa 264-313.

Entrez Gene IDs

55600 (Human); 16429 (Mouse)

Alternate Names

Endothelial lectin HL-1; FLJ20022; Galactofuranose-binding lectin; hIntL; HL-1; intelectin 1 (galactofuranose binding); Intelectin1; Intelectin-1; INTLHL1; ITLN1; Itlna; ITLNITLN-1; LFR; LFRIntestinal lactoferrin receptor; Omentin; Omentin-1