Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Abrilumab Biosimilar) Antibody

Catalog #: MAB10548 Datasheet / COA / SDS

Recombinant Monoclonal Antibody For Research Use Only

Catalog #	Availability	Size / Price	Qty
MAB10548-100
MAB10548-SP

Detection of Integrin alpha 4 beta 7 in Human PBMC by Flow Cytometry.

2 Images

All Integrin alpha 4 beta 7/LPAM-1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha 4 beta 7/LPAM-1 (Research Grade Abrilumab Biosimilar) Antibody Summary

Species Reactivity

Human

Specificity

Detects Human Integrin alpha 4 beta 7/LPAM‑1 in direct ELISAs.This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Abrilumab. This product is for research use only.

Source

Recombinant Monoclonal Human IgG₁ Clone # Hu143

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human Integrin alpha4 beta7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of Integrin alpha 4 beta 7 in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Human Anti-Human Integrin a4 beta 7 (Research Grade Abrilumab Biosimilar) Monoclonal Antibody (Catalog # MAB10548) or (B) no primary antibody, followed by anti-Human IgG APC-conjugated Secondary Antibody (F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (FAB100P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 4 beta 7/LPAM-1

Abrilumab is an anti-human alpha 4 beta 7 integrin heterodimer monoclonal antibody designed for the treatment of inflammatory bowel disease, ulcerative colitis, and Crohn's disease.

Entrez Gene IDs

3676 (Human); 16402 (Mouse)

Alternate Names

269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49d/beta7; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4 beta 7; integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); LPAM1; LPAM-1; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha