Human Insulin Antibody

Name: Human Insulin Antibody
Brand: R&D Systems
SKU: MAB8056
Rating: 5 (1 reviews)

Catalog #: MAB8056 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8056
MAB8056-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Insulin Antibody Summary

Species Reactivity

Human

Specificity

Detects human Insulin in ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 873724

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

The construct consists of the alpha mating factor signal followed by a 10 aa peptide leader, B chain aa 1-29, AAK spacer A chain aa 1-21.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Insulin in Human Pancreas. Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Insulin Monoclonal Antibody (Catalog # MAB8056) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Entrez Gene IDs

3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)

Alternate Names

IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin