Human ING1 Antibody

Catalog #: AF5758 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5758
AF5758-SP

Detection of Human ING1 by Western Blot.

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Human ING1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ING1 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) ING2 and less than 1% cross-reactivity with with rhING3, rhING4, and rhING5 is observed.

Source

Polyclonal Goat IgG

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ING1 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ING1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ING1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry

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ING1 in HeLa Human Cell Line. ING1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human ING1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5758) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

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ING1 in A431 Human Cell Line. ING1 was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human ING1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5758) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry

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ING1 in Human Lung Cancer Tissue. ING1 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using 5 µg/mL Goat Anti-Human ING1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5758) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: ING1

ING1 (Inhibitor of Growth 1; also p33^ING1b and ING1A) is a 32 kDa member of the ING family of tumor suppressors. It is very widely expressed, and participates in multiple cell functions. When bound to p53, ING1 inhibits cell growth; when complexed to GADD45, ING1 enhances DNA nucleotide excision repair; and when associated with HAT, ING1 promotes hyperacetylation of histones H3 and H4. Human ING1 is 279 amino acids (aa) in length. It contains a PIP-PBD region (aa 5‑45), an NLS (aa 142‑158), and a plant homeodomain (PHD) (aa 210‑259). Phosphorylation occurs on Ser126 and Ser199. There are at least three isoform variants. p47^ING1a contains a 190 aa substitution for aa 1‑46, p27^ING1d shows a deletion of aa 2‑45, and p24^ING1c has an alternate start site at Met70. Over aa 213‑327, human ING1 shares 82% aa identity with mouse ING1.

Long Name

Inhibitor of Growth 1

Entrez Gene IDs

3621 (Human); 26356 (Mouse); 306626 (Rat)

Alternate Names

growth inhibitor ING1; growth inhibitory protein ING1; ING1; inhibitor of growth family, member 1; inhibitor of growth protein 1; p24ING1c; p33; p33ING1; p33ING1b; p47; p47ING1a; tumor suppressor ING1