Human IL-6R alpha Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF227
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Human IL-6R alpha Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6R alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) IL‑6, recombinant mouse IL-6, recombinant porcine IL-6, and rhgp130 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
Leu20-Asp358
Accession # P08887
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL-6R alpha (Catalog # 227-SR)

Human IL-6R alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human IL-6R alpha Antibody (Catalog # MAB227R)

Capture Reagent: Human IL-6R alpha Antibody (Catalog # MAB227)

Standard: Recombinant Human IL-6R alpha Protein (Catalog # 227-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6R alpha

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

References
  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al. (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.
Long Name
Interleukin 6 Receptor alpha
Entrez Gene IDs
3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names
CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

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Citations for Human IL-6R alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis.
    Authors: Komori M, Blake A, Greenwood M et al.
    Ann Neurol
  2. Interleukin-6 controls recycling and degradation, but not internalization of its receptors
    Authors: CM Flynn, B Kespohl, T Daunke, Y Garbers, S Düsterhöft, S Rose-John, J Haybaeck, J Lokau, S Aparicio-S, C Garbers
    The Journal of Biological Chemistry, 2021-02-19;0(0):100434.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  3. Exploitation of Elevated Extracellular ATP to Specifically Direct Antibody to Tumor Microenvironment
    Authors: F Mimoto, K Tatsumi, S Shimizu, S Kadono, K Haraya, M Nagayasu, Y Suzuki, E Fujii, M Kamimura, A Hayasaka, H Kawauchi, K Ohara, M Matsushita, T Baba, H Susumu, T Sakashita, T Muraoka, K Aso, H Katada, E Tanaka, K Nakagawa, M Hasegawa, M Ayabe, T Yamamoto, S Tanba, T Ishiguro, T Kamikawa, T Nambu, T Kibayashi, Y Azuma, Y Tomii, A Kato, K Ozeki, N Murao, M Endo, J Kikuta, M Kamata-Sak, M Ishii, K Hattori, T Igawa
    Cell Reports, 2020-12-22;33(12):108542.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Capture
  4. Inhibitory FcgammaRIIb-Mediated Soluble Antigen Clearance from Plasma by a pH-Dependent Antigen-Binding Antibody and Its Enhancement by Fc Engineering.
    Authors: Iwayanagi Y, Igawa T, Maeda A, Haraya K, Wada N, Shibahara N, Ohmine K, Nambu T, Nakamura G, Mimoto F, Katada H, Ito S, Tachibana T, Jishage K, Hattori K
    J Immunol, 2015-08-28;195(7):3198-205.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Development
  5. A disintegrin and metalloprotease 17 dynamic interaction sequence, the sweet tooth for the human interleukin 6 receptor.
    Authors: Dusterhoft S, Hobel K, Oldefest M, Lokau J, Waetzig G, Chalaris A, Garbers C, Scheller J, Rose-John S, Lorenzen I, Grotzinger J
    J Biol Chem, 2014-04-30;289(23):16336-48.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  6. Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa.
    Authors: Wood MW, Breitschwerdt EB, Gookin JL
    Infect. Immun., 2010-11-29;79(2):708-15.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  8. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  9. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001-06-01;14(6):705-14.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  10. Proteolytic Origin of the Soluble Human IL-6R In Vivo and a Decisive Role of N-Glycosylation
    Authors: S Riethmuell, P Somasundar, JC Ehlers, CW Hung, CM Flynn, J Lokau, M Agthe, S Dsterh”ft, Y Zhu, J Gr”tzinger, I Lorenzen, T Koudelka, K Yamamoto, U Pickhinke, R Wichert, C Becker-Pau, M R„disch, A Albrecht, M Hessefort, D Stahnke, C Unverzagt, S Rose-John, A Tholey, C Garbers
    PLoS Biol, 2017-01-06;15(1):e2000080.

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Human IL-6 R alpha Biotinylated Antibody
By Anonymous on 06/01/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human IL-6 receptor alpha was measured in human plasma samples using an immunoassay comprising of this antibody and MAB227. This antibody was used as the detection and the MAB227 was used as the solid phase capture molecule.