Human IL-3R alpha/CD123 Antibody

Name: Human IL-3R alpha/CD123 Antibody
Brand: R&D Systems
SKU: AF841
Rating: 4 (3 reviews)

Catalog #: AF841 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF841
AF841-SP

Detection of Human IL-3R alpha/CD123 by Western Blot.

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Human IL-3R alpha/CD123 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 2% cross‑reactivity with recombinant human (rh) IL-2 sR beta, rhIL-2 sR gamma, and rhIL-6 sR is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑3 R alpha /CD123

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

HDLM‑2 human Hodgkin's lymphoma cell line, TF‑1 human erythroleukemic cell line, and THP‑1 human acute monocytic leukemia cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL-3R alpha/CD123 by Western Blot. Western blot shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, TF‑1 human erythroleukemic cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-3R alpha/CD123 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF841) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for IL-3R alpha/CD123 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3R alpha

Interleukin 3 is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity human IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit does not bind IL-3 by itself but is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF. Both the alpha and the beta subunits are members of the cytokine receptor superfamily.

References

Ogorochi, T. and A. Miyajima (1994) in Guidebook to Cytokines and Their Receptors, N.A. Nicola, ed. Oxford University, New York p. 40.

Long Name

Interleukin 3 Receptor alpha

Entrez Gene IDs

3563 (Human); 16188 (Mouse); 102138639 (Cynomolgus Monkey)

Alternate Names

CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 R alpha; IL-3 receptor subunit alpha; IL3R alpha; IL-3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174