Home / IL-31 / Human IL-31 Antibody

Human IL-31 Antibody

Catalog #: AF2824
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2824
AF2824-SP
IL‑31 in Human PBMCs.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-31 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑31 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL-31 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-31
Ser24-Thr164
Accession # Q6EBC2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑31 (Catalog # 2824-IL)
Immunocytochemistry
5-15 µg/mL
See below

Human IL-31 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human IL‑31 Biotinylated Antibody (Catalog # BAF2824)

Standard: Recombinant Human IL-31 Protein (Catalog # 2824-IL)

Neutralization
Measured by its ability to neutralize IL‑31-induced STAT3 activation in the U‑87 MG human glioblastoma/astrocytoma cell line. 1 μg/mL of this antibody can completely neutralize rhIL-31 (5 ng/mL) induced STAT3 activation in U-87 MG cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-31 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑31 in Human PBMCs. IL-31 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Human IL-31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2824) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-31

Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein (1, 2). The mature region shows four alpha -helices which would be expected to show a typical up‑up‑down‑down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region (1). IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL‑31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ) (2‑6). In the complex, IL-31 directly binds to GPL, not OSM R (2, 3). IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway (2‑5). Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable (2, 3). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFN-gamma on monocytes (1). Studies using transgenic mice indicate that IL-31 may contribute to the pruritis (itching) associated with nonatopic dermatitis (1, 7).

References
  1. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752.
  2. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
  3. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
  4. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
  5. Ghilardi, N. et al. (2002) J. Biol. Chem 277:16831.
  6. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
  7. Takaoka, A. et al. (2005) Eur. J. Pharmacol. 516:180.
Long Name
Interleukin 31
Entrez Gene IDs
386653 (Human); 76399 (Mouse)
Alternate Names
IL31; IL-31; IL-31interleukin-31; interleukin 31

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human IL-31 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system
    Authors: A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai
    Nature Communications, 2022-10-14;13(1):6078.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Effect of interleukin‑31 on septic shock through regulating inflammasomes and interleukin‑1 beta
    Authors: Xuyun Gu, Chen Wei, Xishan Zhu, Feiping Lu, Bo Sheng, Xuefeng Zang
    Experimental and Therapeutic Medicine

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IL-31 Antibody

There are currently no reviews for this product. Be the first to review Human IL-31 Antibody and earn rewards!

Have you used Human IL-31 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review