Home / IL-28 R alpha/IFN-lambda R1 / Human IL-28 R alpha /IFN-lambda  R1 Antibody

Human IL-28 R alpha /IFN-lambda  R1 Antibody

Catalog #: AF5260
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5260
AF5260-SP
Detection of Human IL‑28 R alpha /IFN‑ lambda R1 by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-28 R alpha /IFN-lambda  R1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-28 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse
IL‑28 R alpha is observed.
Source
Polyclonal Sheep IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-28 Ra/IFN-? R1 antibody by Western Blot. View Larger

Detection of Human IL‑28 R alpha /IFN‑ lambda R1 by Western Blot. Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 2 µg/mL of Human IL-28 Ra/IFN-lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-28 Ra/IFN-lambda R1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Flow Cytometry Detection of IL-28 Ra/IFN-? R1 antibody in Daudi Human Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑28 R alpha /IFN‑ lambda R1 in Daudi Human Cell Line by Flow Cytometry. Daudi human Burkitt's lymphoma cell line was stained with Human IL-28 Ra/IFN-lambda R1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5260, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-28 R alpha/IFN-lambda R1

IL-28 R alpha (IL-28 receptor alpha subunit; also named interferon-lambda R1) is a type I transmembrane glycoprotein that is the cytokine receptor family 2 member 12
(CRF2-12) (1-4). It pairs with the IL-10 receptor beta subunit (IL-10 R beta, CRF2-4) to form the IL-28 R (1-4). Each subunit of this receptor can interact with the
interferon-like cytokines (type III interferons) IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3) or IL-29 (IFN-lambda 1) (1-4). Human IL-28 R alpha cDNA encodes a 520 amino acid (aa) protein with a 20 aa signal peptide, a 208 aa extracellular domain (ECD) with a fibronectin type III motif and four potential N-glycosylation sites, a 21 aa transmembrane sequence, and a proline-rich and acidic 271 aa cytoplasmic domain. Eight isoforms have been sequenced, but their significance is unknown (3, 5). Isoforms of 211 and 244 aa appear to lack transmembrane sequences. These and other isoforms of 491, 437, 322, 283 and 184 aa have alternate N- or C-termini, or lack an internal sequence (aa 268-296). The mature human IL-28 R alpha ECD shares 67%, 66%, 78% and 74% aa sequence identity with mouse, rat, canine and bovine IL-28 R alpha, respectively. Some cross-species reactivity has been shown (6). IL-28 R is constitutively expressed in most tissues, but its ligands are mainly produced by antigen presenting cells in response to viruses and their products (2-6). Signaling through IL-28 R alpha is similar to that of receptors for type I IFNs, including tyrosine phosphorylation, activation of JAK tyrosine kinases, STAT phosphorylation and formation of the IFN-stimulated gene factor 3 (ISGF-3) transcription factor complex
(1-7). This signaling pathway induces antiviral activity and up-regulates MHC class I antigen expression (2-7). Anti-proliferative activity has also been shown for
IL-28/IL-28 R (7).

References
  1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
  2. Meager, A. et al. (2005) Cytokine 31:109.
  3. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  4. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  5. Entrez Accession # NP_775088, EAW95113, EAW95115, EAW95116, AAI39723, AAI01409.
  6. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
  7. Dumoutier, L. et al. (2004) J. Biol. Chem. 279:32269.
Long Name
Interleukin 28 Receptor alpha
Entrez Gene IDs
163702 (Human); 242700 (Mouse); 362628 (Rat)
Alternate Names
class II cytokine receptor CRF2/12; CRF2/12; CRF2-12; Cytokine receptor class-II member 12; Cytokine receptor family 2 member 12; IFN-lambda R1; IFN-lambda receptor 1; IFN-lambda-R1; IFNLR; IFNLR1; IL-28 R alpha; IL-28 receptor subunit alpha; IL28R alpha; IL-28R1; IL28RA; IL-28Ra; IL-28R-alpha; Interferon lambda receptor 1; interferon lambda, receptor 1; interleukin 28 receptor A; interleukin 28 receptor, alpha (interferon, lambda receptor); interleukin 28 receptor, alpha; interleukin or cytokine receptor 2; interleukin-28 receptor subunit alpha; LICR2; Likely interleukin or cytokine receptor 2

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Citations for Human IL-28 R alpha /IFN-lambda  R1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Replication of human norovirus RNA in mammalian cells reveals a lack of interferon response
    J Virol, 2016-09-12;0(0):.
  2. Casein kinase 1? mediates degradation of receptors for type I and type II interferons caused by hemagglutinin of influenza A virus
    Authors: C Xia, JJ Wolf, M Vijayan, CJ Studstill, W Ma, B Hahm
    J. Virol., 2018-03-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A paradox of transcriptional and functional innate interferon responses of human intestinal enteroids to enteric virus infection
    Authors: K Saxena, LM Simon, XL Zeng, SE Blutt, SE Crawford, NP Sastri, UC Karandikar, NJ Ajami, NC Zachos, O Kovbasnjuk, M Donowitz, ME Conner, CA Shaw, MK Estes
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-09;114(4):E570-E579.
    Species: Primate - Chlorocebus aethiops (African Green Monkey)
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Type III IFN interleukin-28 mediates the antitumor efficacy of oncolytic virus VSV in immune-competent mouse models of cancer.
    Authors: Wongthida P, Diaz RM, Galivo F
    Cancer Res., 2010-05-18;70(11):4539-49.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization

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