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Human IL-24 Antibody

Catalog #: AF1965
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1965
AF1965-SP
Cell Proliferation Induced by IL‑24 and Neutralization by Human IL‑24 Antibody.
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Citations (13)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-24 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-24 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑24
Gln50-Leu206
Accession # Q2YHE5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑24 (Catalog # 1965-IL)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells fixed with paraformaldehyde and permeabilized with saponin
Neutralization
Measured by its ability to neutralize IL‑24-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically < 0.6 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑24.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑24 and Neutralization by Human IL‑24 Antibody. View Larger

Cell Proliferation Induced by IL‑24 and Neutralization by Human IL‑24 Antibody. Recombinant Human IL-24 (Catalog # 1965-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 Ra and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-24 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1965). The ND50 is typically < 0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-24

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids that contains a 48 amino acid (aa) signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35-40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4+ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1 beta, IL‑12, and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta, which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta, which is shared with IL-20.

References
  1. Jiang, H. et al. (1995) Oncogene 11:2477.
  2. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
  3. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
  4. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
  5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  6. Chen, J. et al. (2003) Molec. Ther. 8:220.
Long Name
Interleukin 24
Entrez Gene IDs
11009 (Human); 93672 (Mouse); 170819 (Rat)
Alternate Names
C49A; FISP; IL10B; IL24; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein

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Citations for Human IL-24 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Identification of targets of IL-13 and STAT6 signaling in polycystic kidney disease
    Authors: Erin E. Olsan, Jonathan D. West, Jacob A. Torres, Nicholas Doerr, Thomas Weimbs
    American Journal of Physiology-Renal Physiology
  2. Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo
    Authors: QUNFENG MA, BANGMING JIN, YAO ZHANG, YINAN SHI, CHI ZHANG, DAN LUO et al.
    Oncology Reports
  3. Hiltonol Cocktail Kills Lung Cancer Cells by Activating Cancer-Suppressors, PKR/OAS, and Restraining the Tumor Microenvironment
    Authors: SC Chang, BX Zhang, EC Su, WC Wu, TH Hsieh, AM Salazar, YK Lin, JL Ding
    International Journal of Molecular Sciences, 2021-02-05;22(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Effects of melanoma differentiation associated gene-7 (MDA-7/IL-24) on apoptosis of liver cancer cells via regulating the expression of B-cell lymphoma-2
    Authors: Q Mo, L Liu, G Bao, T Li
    Oncol Lett, 2019-05-02;18(1):29-34.
    Species: Human
    Sample Types: Cell Lysate, Cell Lysates
    Applications: Western Blot
  5. Enhanced tumor growth inhibition by mesenchymal stem cells derived from iPSCs with targeted integration of interleukin24 into rDNA loci
    Authors: B Liu, F Chen, Y Wu, X Wang, M Feng, Z Li, M Zhou, Y Wang, L Wu, X Liu, D Liang
    Oncotarget, 2017-06-20;8(25):40791-40803.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Cell Lysates, Whole Tissue
    Applications: ELISA Development, IHC, Western Blot
  6. Expansion of parasite-specific CD4+ and CD8+ T cells expressing IL-10 superfamily cytokine members and their regulation in human lymphatic filariasis.
    Authors: Anuradha R, George P, Hanna L, Kumaran P, Chandrasekaran V, Nutman T, Babu S
    PLoS Negl Trop Dis, 2014-04-03;8(4):e2762.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. T cell-derived microvesicles induce mast cell production of IL-24: relevance to inflammatory skin diseases.
    Authors: Shefler I, Pasmanik-Chor M, Kidron D, Mekori Y, Hershko A
    J Allergy Clin Immunol, 2013-06-12;133(1):217-24.e1-3.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Induction of phenotype modifying cytokines by FERMT1 mutations.
    Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
    Hum. Mutat., 2011-03-01;32(4):397-406.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells.
    Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A
    Blood, 2009-11-24;115(9):1718-26.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Systemic cancer gene therapy using adeno-associated virus type 1 vector expressing MDA-7/IL24.
    Authors: Tahara I, Miyake K, Hanawa H, Kurai T, Hirai Y, Ishizaki M, Uchida E, Tajiri T, Shimada T
    Mol. Ther., 2007-06-05;15(10):1805-11.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  11. Crystal structure of the labile complex of interleukin 24 (IL-24) with the extracellular domains of IL-22R1 and IL-20R2
    Authors: Jacek Lubkowski, Cem Sonmez, Sergey V. Smirnov, Andriy Anishkin, Sergei V. Kotenko, Alexander Wlodawer
    The Journal of Immunology
  12. Oncolytic adenovirus-mediated mda-7/IL-24 expression suppresses osteosarcoma growth and enhances sensitivity to doxorubicin
    Authors: ZONGMING LIU, LIBO XU, HONGPING YUAN, YANG ZHANG, XIAONA ZHANG, DONGXU ZHAO
    Molecular Medicine Reports
  13. A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells
    Authors: QUNFENG MA, XUEFENG DENG, BANGMING JIN, YAO ZHANG, DAN LUO, HEYU SONG et al.
    Oncology Reports

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Human IL-24 Antibody
By Anonymous on 11/04/2016
Application: IHC Sample Tested: Adult lung Species: Human
Immunohistochemistry Human IL-24 Antibody AF1965