Human IL-23R Fluorescein-conjugated Antibody Summary
Gly24-Ile354
Accession # Q5VWK5
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑23 R in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 200 ng/mL Calcium Ionomycin, 200 ng/mL LPS, 20 ng/mL Recombinant Human IL-23 (Catalog # 1290-IL), and 40 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) overnight to induce Th17 development were stained with Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human IL-23 R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB14001F) or (B) Mouse IgG2BFluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-23R
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.
- Oppmann, B. et al. (2000) Immunity 13:715.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Parham, C. et al. (2002) J. Immunol. 168:5448.
- Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Product Datasheets
Citations for Human IL-23R Fluorescein-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Blockade of PGK1 and ALDOA enhances bilirubin control of Th17 cells in Crohn’s disease
Authors: Marta Vuerich, Na Wang, Jonathon J. Graham, Li Gao, Wei Zhang, Ahmadreza Kalbasi et al.
Communications Biology
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Endogenous antisense RNA curbs CD39 expression in Crohn's disease
Authors: RP Harshe, A Xie, M Vuerich, LA Frank, B Gromova, H Zhang, RJ Robles, S Mukherjee, E Csizmadia, E Kokkotou, AS Cheifetz, AC Moss, SK Kota, SC Robson, MS Longhi
Nat Commun, 2020-11-18;11(1):5894.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Functional significance of MAIT cells in psoriatic arthritis
Authors: SK Raychaudhu, C Abria, A Mitra, SP Raychaudhu
Cytokine, 2019-09-18;125(0):154855.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The activation status of human macrophages presenting antigen determines the efficiency of Th17 responses.
Authors: Arnold C, Gordon P, Barker R, Wilson H
Immunobiology, 2015-01-01;220(1):10-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Boswellic acids reduce Th17 differentiation via blockade of IL-1beta-mediated IRAK1 signaling.
Authors: Sturner K, Verse N, Yousef S, Martin R, Sospedra M
Eur J Immunol, 2014-02-16;44(4):1200-12.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis.
Authors: Bowness P, Ridley A, Shaw J
J. Immunol., 2011-01-19;186(0):2672.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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