Human IL-23R APC-conjugated Antibody Summary
Gly24-Ile354
Accession # Q5VWK5
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑23 R in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) cultured with plate-bound 5 μg/mL anti-Human CD3, 2 μg/mL anti-Human CD28 (AF-342-PB), 20 ng/mL Recombinant Human IL-2 (202-GMP), 10 ng/mL TGF-beta (100-B), 20 ng/mL Recombinant Human IL-23 (1290-IL), 40 ng/mL Recombinant Human IL-6 (206-IL), 10 ng/mL Recombinant Human IL-1 beta (201-LB), and 10 μg/mL anti-Human IFN-gamma (AF-285-NA) for 7 days to induce Th17 development or (B) resting, were stained with Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P) and Mouse Anti-Human IL-23 R APC-conjugated Monoclonal Antibody (Catalog # FAB14001A). Quadrant markers were set based on Mouse IgG2B Isotype Control (IC0041A). Staining was performed using our Staining Membrane-associated Proteins protocol.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-23R
Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.
- Oppmann, B. et al. (2000) Immunity 13:715.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Parham, C. et al. (2002) J. Immunol. 168:5448.
- Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Product Datasheets
Citations for Human IL-23R APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Pathogenic function of bystander-activated memory-like CD4+ T cells in autoimmune encephalomyelitis
Authors: HG Lee, JU Lee, DH Kim, S Lim, I Kang, JM Choi
Nat Commun, 2019-02-12;10(1):709.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-11 Induces Th17 Cell Responses in Patients with Early Relapsing-Remitting Multiple Sclerosis.
Authors: Zhang X, Tao Y, Chopra M, Dujmovic-Basuroski I, Jin J, Tang Y, Drulovic J, Markovic-Plese S
J Immunol, 2015-04-20;194(11):5139-49.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Age and CD161 expression contribute to inter-individual variation in interleukin-23 response in CD8+ memory human T cells.
Authors: Shen H, Zhang W, Abraham C, Cho J
PLoS ONE, 2013-03-01;8(3):e57746.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
Authors: Madrid, Alexis S, Ganem, Don
J Virol, 2012-06-06;86(16):8693-704.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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