Human IL-18 R beta /IL-1 R7 PE-conjugated Antibody

Catalog #: FAB118P
7 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB118P-100
FAB118P-025

Detection of IL‑18 R beta /IL‑1 R7 in KG‑1 Human Cell Line by Flow Cytometry.

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Citations (7)

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Human IL-18 R beta /IL-1 R7 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑18 R beta /IL‑1 R7 in direct ELISAs and Western blots. In direct ELISAs, 15% cross-reactivity with recombinant human (rh) IL‑1 RII is observed and 2% cross-reactivity with rhIL-1 R9 is observed. Also in direct ELISAs, no cross-reactivity with rhIL-1 RI, rhIL‑1 RacP/IL‑1 R3, rhIL-1 R4, rhIL-18 R alpha /IL-1 R5, rhIL-1 R6, rhIL-1 R8, or recombinant mouse (rm) IL-18 R beta /IL-1 R7 is observed. In Western blots, approximately 100% cross-reactivity with rmIL-18 R beta is observed and no cross-reactivity with rhIL-18 R alpha /IL-1 R5, rhIL‑1 RacP/IL‑1 R3, rhIL-1 RI, rhIL-1 RII, or rhIL-1 Rrp2/IL-1 R6 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 132029

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑18 R beta /IL‑1 R7
Met1-Arg356
Accession # O95256

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑18 R beta /IL‑1 R7 in KG‑1 Human Cell Line by Flow Cytometry. KG-1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL-18 R beta /IL-1 R7 PE-conjugated Mono-clonal Antibody (Catalog # FAB118P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL‑18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

References

Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Long Name

Interleukin 18 Receptor beta

Entrez Gene IDs

8807 (Human); 16174 (Mouse)

Alternate Names

Accessory protein-like; AcPL; CD218 antigen-like family member B; CD218b antigen; CD218b; CDw218b; IL-1 R7; IL-18 R beta; IL-18 receptor accessory protein; IL-18 receptor beta; IL18R beta; IL-18RAcP; IL18RAP; IL18RB; IL-18Rb; IL-18Rbeta; IL-18R-beta; IL-1R accessory protein-like; IL1R7; IL-1R7; IL-1R-7; IL-1RAcPL; interleukin 18 receptor accessory protein; Interleukin-1 receptor 7; interleukin-18 receptor accessory protein; Interleukin-18 receptor accessory protein-like; Interleukin-18 receptor beta; MGC120589; MGC120590

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human IL-18 R beta /IL-1 R7 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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KIR+CD8+ and NKG2A+CD8+ Tï¿½cells are distinct innate-like populations in humans
Authors: SJ Choi, JY Koh, MS Rha, IH Seo, H Lee, S Jeong, SH Park, EC Shin
Cell Reports, 2023-03-09;42(3):112236.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Differential IL-12 signaling induces human natural killer cell activating receptor-mediated ligand-specific expansion
Authors: Avishai Shemesh, Harry Pickering, Kole T. Roybal, Lewis L. Lanier
Journal of Experimental Medicine
Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
J Immunol, 2015-07-31;195(5):2141-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
TCR-dependent sensitization of human gamma δ T cells to non-myeloid IL-18 in cytomegalovirus and tumor stress surveillance
Authors: Florent Guerville, Sophie Daburon, Romain Marlin, Lydia Lartigue, Severine Loizon, Vincent Pitard et al.
OncoImmunology
Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression.
Authors: Uzan B, Poglio S, Gerby B, Wu C, Gross J, Armstrong F, Calvo J, Cahu X, Deswarte C, Dumont F, Passaro D, Besnard-Guerin C, Leblanc T, Baruchel A, Landman-Parker J, Ballerini P, Baud V, Ghysdael J, Baleydier F, Porteu F, Pflumio F
EMBO Mol Med, 2014-04-06;6(6):821-34.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Increased expression of Interleukin-18 receptor in blood cells of subjects with Mild Cognitive Impairment and Alzheimer's disease.
Cytokine, 2012-11-30;61(2):360-3.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis.
Authors: De Jager W, Vastert SJ, Beekman JM, Wulffraat NM, Kuis W, Coffer PJ, Prakken BJ
Arthritis Rheum., 2009-09-01;60(9):2782-93.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry