Human IL-17/IL-17A APC-conjugated Antibody

Catalog #: IC3171A
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
IC3171A-100
IC3171A-025

Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry.

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All IL-17/IL-17A Antibodies

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-17/IL-17A APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-17 in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) IL-17A/IL-17F heterodimer is observed. No cross-reactivity with recombinant mouse IL-17, recombinant canine Il-17, rhIL-17B, rhIL-17C, rhIL-17D, rhIL-17E, or rhIL-17F is observed.

Source

Monoclonal Mouse IgG₁ Clone # 41802

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL-17
Ile20-Ala155
Accession # Q16552

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-17/IL-17A antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of IL‑17/IL‑17A in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-17/IL-17A APC-conjugated Monoclonal Antibody (Catalog # IC3171A) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17/IL-17A

Interleukin-17 (IL-17) is a pro-inflammatory cytokine secreted by activated T cells. It is the prototype member of the IL-17 family that also includes IL-17B, C, D, E, and F.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-17/IL-17A APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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The Expression of IL-17, in Chronic Spontaneous Urticaria Is Linked to Semaphorin5A
Authors: M Lobna, E Nasren, S Adi, B Jacob, GA Maria, M Marcus, V Zahava
Biomolecules, 2021-03-02;11(3):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
TLR7/8 adjuvant overcomes newborn hyporesponsiveness to pneumococcal conjugate vaccine at birth
Authors: DJ Dowling, SD van Haren, A Scheid, I Bergelson, D Kim, CJ Mancuso, W Foppen, A Ozonoff, L Fresh, TB Theriot, AA Lackner, RN Fichorova, D Smirnov, JP Vasilakos, JM Beaurline, MA Tomai, CC Midkiff, X Alvarez, JL Blanchard, MH Gilbert, PP Aye, O Levy
JCI Insight, 2017-03-23;2(6):e91020.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry
Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization
Authors: Ofer Levy
J. Immunol., 2016-10-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Sunitinib indirectly enhanced anti-tumor cytotoxicity of cytokine-induced killer cells and CD3(+)CD56(+) subset through the co-culturing dendritic cells.
Authors: Wongkajornsilp A, Wamanuttajinda V, Kasetsinsombat K, Duangsa-ard S, Sa-ngiamsuntorn K, Hongeng S, Maneechotesuwan K
PLoS ONE, 2013-11-13;8(11):e78980.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
IL-21 and TGF-beta are required for differentiation of human T(H)17 cells.
Authors: Yang L, Anderson DE, Baecher-Allan C, Hastings WD, Bettelli E, Oukka M, Kuchroo VK, Hafler DA
Nature, 2008-05-11;454(7202):350-2.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry