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Human IL-15R alpha Antibody

Catalog #: AF247
14 Citations 2 Reviews Datasheet / COA / SDS
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AF247
AF247-SP
IL‑15 R alpha in HDLM‑2 Human Cell Line.
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Citations (14)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-15R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human IL-2 R gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-15 R alpha Ile31-Thr172 Accession # EAW86418
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑15 R alpha Fc Chimera (Catalog # 147-IR)
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with PHA
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
1-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑15 R alpha -mediated inhibition of proliferation in the CTLL‑2 mouse cytotoxic T cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑15 R alpha Fc Chimera and 2 ng/mL Recombinant Human IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL-15 Ra antibody in HDLM-2 Human Cell Line by Immunocytochemistry (ICC). View Larger

IL‑15 R alpha in HDLM‑2 Human Cell Line. IL-15 Ra was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry IL‑15 Ra antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

IL‑15 R alpha in Human Prostate Cancer Tissue. IL‑15 Ra was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human IL‑15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization IL‑15 R alpha Inhibition of IL‑15-dependent Cell Proliferation and Neutralization by Human IL‑15 R alpha Antibody. View Larger

IL‑15 R alpha Inhibition of IL‑15-dependent Cell Proliferation and Neutralization by Human IL‑15 R alpha Antibody. Recombinant Human IL-15 Ra Fc Chimera (Catalog # 147-IR) inhibits Recombinant Human IL-15 (Catalog # 247-IL) induced proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-15 (2 ng/mL) activity elicited by Recombinant Human IL-15 Ra Fc Chimera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-15 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF247). The ND50 is typically 1-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL-15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of IL-15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 amino acid (aa) protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain, and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References
  1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
  3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
  4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
Long Name
Interleukin 15 Receptor alpha
Entrez Gene IDs
3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)
Alternate Names
CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

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Citations for Human IL-15R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Priming of Human Resting NK Cells by Autologous M1 Macrophages via the Engagement of IL-1beta, IFN-beta, and IL-15 Pathways.
    Authors: Mattiola I, Pesant M, Tentorio P, Molgora M, Marcenaro E, Lugli E, Locati M, Mavilio D
    J Immunol, 2015-08-14;195(6):2818-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC, Neutralization
  2. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
    Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
    Gene Ther, 2014-10-02;22(1):76-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Interleukin-15 enhances cellular proliferation and upregulates CNS homing molecules in pre-B acute lymphoblastic leukemia.
    Authors: Williams M, Yousafzai Y, Cox C, Blair A, Carmody R, Sai S, Chapman K, McAndrew R, Thomas A, Spence A, Gibson B, Graham G, Halsey C
    Blood, 2014-04-03;123(20):3116-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Regulation of CD4(+)NKG2D(+) Th1 cells in patients with metastatic melanoma treated with sorafenib: role of IL-15Ralpha and NKG2D triggering.
    Authors: Romero A, Chaput N, Poirier-Colame V, Rusakiewicz S, Jacquelot N, Chaba K, Mortier E, Jacques Y, Caillat-Zucman S, Flament C, Caignard A, Messaoudene M, Auperin A, Vielh P, Dessen P, Porta C, Mateus C, Ayyoub M, Valmori D, Eggermont A, Robert C, Zitvogel L
    Cancer Res, 2013-11-06;74(1):68-80.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells.
    Authors: Muller J, Waldmann T, Kruhlak M, Dubois S
    J Biol Chem, 2012-10-16;287(48):40328-38.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development
  6. Interleukin-15 plays a central role in human kidney physiology and cancer through the gamma c signaling pathway.
    Authors: Giron-Michel J, Azzi S, Khawam K, Mortier E, Caignard A, Devocelle A, Ferrini S, Croce M, Francois H, Lecru L, Charpentier B, Chouaib S, Azzarone B, Eid P
    PLoS ONE, 2012-02-21;7(2):e31624.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. EBV-associated mononucleosis does not induce long-term global deficit in T-cell responsiveness to IL-15.
    Authors: Giron-Michel J, Menard F, Negrini S, Devocelle A, Azzarone B, Besson C
    Blood, 2009-03-04;113(19):4541-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Increased IL-15 production is associated with higher susceptibility of memory CD4 T cells to simian immunodeficiency virus during acute infection.
    Authors: Eberly MD, Kader M, Hassan W, Rogers KA, Zhou J, Mueller YM, Mattapallil MJ, Piatak M, Lifson JD, Katsikis PD, Roederer M, Villinger F, Mattapallil JJ
    J. Immunol., 2009-02-01;182(3):1439-48.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Human T cells constitutively express IL-15 that promotes ex vivo T cell homeostatic proliferation through autocrine/juxtacrine loops.
    Authors: Miranda-Carus ME, Benito-Miguel M, Llamas MA, Balsa A, Martin-Mola E
    J. Immunol., 2005-09-15;175(6):3656-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Renal cells express a functional interleukin-15 receptor.
    Authors: Tejman-Yarden N, Zlotnik M, Lewis E, Etzion O, Chaimovitz C, Douvdevani A
    Nephrol. Dial. Transplant., 2005-01-25;20(3):516-23.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  11. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
    Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
    Proc. Natl. Acad. Sci. U.S.A., 2004-11-09;101(47):16606-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling.
    Authors: Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M
    Leuk. Res., 2004-10-01;28(10):1023-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004-07-15;173(2):1463-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Cytokine profile of the rheumatoid nodule suggests that it is a Th1 granuloma.
    Authors: Hessian PA, Highton J, Kean A, Sun CK, Chin M
    Arthritis Rheum., 2003-02-01;48(2):334-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human IL-15R alpha Antibody

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Human IL-15R alpha Antibody
By Anonymous on 06/09/2020
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody in an in-house ELISA along with mAb and protein standard (147-IR-100) to quantify IL-15R alpha in human serum and plasma. This combination could not detect IL-15R alpha in our samples but generated a good standard curve.

ELISA Human IL-15R alpha Antibody AF247
Human IL-15R alpha Antibody
By Anonymous on 05/01/2020
Application: ELISA Sample Tested: Serum Species: Human

Ab was used as capture antibody in human serum ELISA

ELISA Human IL-15R alpha Antibody AF247