Home / IL-10 R beta / Human IL-10 R beta Antibody

Human IL-10 R beta Antibody

Catalog #: AF874
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF874
AF874-SP
IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R beta Antibody.
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Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-10 R beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human
(rh) IL‑20 R alpha, rhIL-10 R, and rhIL-20 R beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-10 R beta
Met20-Ser220
Accession # Q08334
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.20 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑10 R beta Fc Chimera (Catalog # 874-RB)
Flow Cytometry
2.5 µg/106 cells
Human whole blood monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑10 R beta -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC). Ralph, P. et al. (1991) J. Immunology 148:808. The Neutralization Dose (ND50) is typically 2-6 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25 ng/mL LPS.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R beta Antibody. View Larger

IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R beta Antibody. Recombinant Human IL-10 (Catalog # 217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta /IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secretion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF874). The ND50 is typically 2-6 µg/mL in the presence of LPS (0.25 ng/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R beta

IL-10, also known as IL-10 R2, mediates its biological activities via binding to a heteromeric receptor complex consisting of two distinct type II cytokine receptor subunits, the ligand binding IL-10 R alpha and the IL-10 R beta which does not bind IL-10 by itself but is required for signal transduction. The cDNA for human IL-10 R beta encodes a 325 amino acid (aa) residue type I transmembrane precursor protein with a 20 aa signal sequence, a 200 aa extracellular region, a 29 aa transmembrane segment, and a short 76 aa cytoplasmic domain. Within the extracellular region, there are two 100 aa subdomains that resemble the constant region of immunoglobulins. This structural motif is responsible for the alternative designation of IL-10 R beta as CRF2-4 (the 4th member of the cytokine receptor family class II/2). Human and mouse IL-10 R beta share approximately 69% aa sequence identity. Binding of the non-covalent IL-10 dimer to two IL-10 R alpha chains recruits two
IL‑10 R beta chains resulting in the activation and phosphorylation of the signaling cascade involving JAK1, TYK2, and STAT3. IL-10 R beta  is expressed ubiquitously.
IL‑10 R beta is also a component of the IL-22 receptor complex consisting of the IL-10 R beta chain and IL-22 R, another type II cytokine receptor family member.

References
  1. Donnelly, R.P. et al. (2004) J. Leukoc. Biol. 76:314.
  2. Donnelly, R.P. et al. (1999) J. Interferon Cytokine Res. 19:563.
  3. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  4. Liu, Y. et al. (1994) J. Immunol. 152:1821.
  5. Lutfalla, G. et al. (1993) Genomics 16:366.
  6. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Long Name
Interleukin 10 Receptor beta
Entrez Gene IDs
3588 (Human); 16155 (Mouse)
Alternate Names
CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 R beta; IL-10 receptor subunit beta; IL10R beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10Rb; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4

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Citations for Human IL-10 R beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Replication of human norovirus RNA in mammalian cells reveals a lack of interferon response
    J Virol, 2016-09-12;0(0):.
  2. IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling
    Authors: AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo
    J. Immunol., 2017-10-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Trisomy 21 consistently activates the interferon response
    Elife, 2016-07-29;5(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. T(H)17 cells promote microbial killing and innate immune sensing of DNA via interleukin 26
    Authors: Stephan Meller, Jeremy Di Di Domizio, Kui S Voo, Heike C Friedrich, Georgios Chamilos, Dipyaman Ganguly et al.
    Nature Immunology
  5. HCV infection selectively impairs type I but not type III IFN signaling.
    Authors: Chandra P, Bao L, Song K, Aboulnasr F, Baker D, Shores N, Wimley W, Liu S, Hagedorn C, Fuchs S, Wu T, Balart L, Dash S
    Am J Pathol, 2013-11-09;184(1):214-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity
    Authors: Noelle D. Brodeur, Juliet V. Spencer
    Virus Research
  7. Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
    Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
    Genes Immun., 2009-10-01;10(8):702-14.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Biological activity of interleukins-28 and -29: comparison with type I interferons.
    Authors: Meager A, Visvalingam K, Dilger P, Bryan D, Wadhwa M
    Cytokine, 2005-07-21;31(2):109-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Cutting edge: IL-26 signals through a novel receptor complex composed of IL-20 receptor 1 and IL-10 receptor 2.
    Authors: Sheikh F, Baurin VV, Lewis-Antes A, Shah NK, Smirnov SV, Anantha S, Dickensheets H, Dumoutier L, Renauld JC, Zdanov A, Donnelly RP, Kotenko SV
    J. Immunol., 2004-02-15;172(4):2006-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Abnormal interleukin 10Ralpha expression contributes to the maintenance of elevated cyclooxygenase-2 in non-small cell lung cancer cells.
    Authors: Heuze-Vourc&amp;apos;h N
    Cancer Res., 2003-02-15;63(4):766-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. IL-28, IL-29 and their class II cytokine receptor IL-28R.
    Authors: Sheppard P, Kindsvogel W, Xu W, Henderson K, Schlutsmeyer S, Whitmore TE, Kuestner R, Garrigues U, Birks C, Roraback J, Ostrander C, Dong D, Shin J, Presnell S, Fox B, Haldeman B, Cooper E, Taft D, Gilbert T, Grant FJ, Tackett M, Krivan W, McKnight G, Clegg C, Foster D, Klucher KM
    Nat. Immunol., 2002-12-02;4(1):63-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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