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Human IL-10 Antibody

Catalog #: MAB2171
17 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2171-SP
MAB2171-100
MAB2171-500
Detection of Human IL‑10 by Western Blot.
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Product Details
Citations (17)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑10.
Source
Monoclonal Mouse IgG2B Clone # 25209
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
5 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9-2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.005-0.03 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-10 antibody by Western Blot. View Larger

Detection of Human IL‑10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Human IL‑10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171). The ND50 is typically 0.005-0.03 µg/mL.

Block/ Neutralize Detection of Human IL-10 by Block/Neutralize View Larger

Detection of Human IL-10 by Block/Neutralize S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha, IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or absence of anti-IL-10 antibodies (Ab). Anti IL-10 Ab was used at 100 ng/ml. The mRNA level was normalized to that of the reference gene peptidylpropylisomerase B (PPIB) and then compared to the control group. Black columns represent controls (cells without any lipopolysaccharide (LPS)), white columns represent LPS-unprimed cells (only stimulated once with 100 ng/ml LPS) and grey columns represent LPS-primed cells (stimulated twice: 2 ng/ml followed by 100 ng/ml). †p<0.01, Wilcoxon paired test. Median (+/− interquartile range) data from 9 independent experiments are given. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24956170), licensed under a CC-BY license. Not internally tested by R&D Systems.

Block/ Neutralize Detection of Human IL-10 by Block/Neutralize View Larger

Detection of Human IL-10 by Block/Neutralize S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha, IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or absence of anti-IL-10 antibodies (Ab). Anti IL-10 Ab was used at 100 ng/ml. The mRNA level was normalized to that of the reference gene peptidylpropylisomerase B (PPIB) and then compared to the control group. Black columns represent controls (cells without any lipopolysaccharide (LPS)), white columns represent LPS-unprimed cells (only stimulated once with 100 ng/ml LPS) and grey columns represent LPS-primed cells (stimulated twice: 2 ng/ml followed by 100 ng/ml). †p<0.01, Wilcoxon paired test. Median (+/− interquartile range) data from 9 independent experiments are given. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24956170), licensed under a CC-BY license. Not internally tested by R&D Systems.

Block/ Neutralize Detection of Human IL-10 by Block/Neutralize View Larger

Detection of Human IL-10 by Block/Neutralize S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha, IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or absence of anti-IL-10 antibodies (Ab). Anti IL-10 Ab was used at 100 ng/ml. The mRNA level was normalized to that of the reference gene peptidylpropylisomerase B (PPIB) and then compared to the control group. Black columns represent controls (cells without any lipopolysaccharide (LPS)), white columns represent LPS-unprimed cells (only stimulated once with 100 ng/ml LPS) and grey columns represent LPS-primed cells (stimulated twice: 2 ng/ml followed by 100 ng/ml). †p<0.01, Wilcoxon paired test. Median (+/− interquartile range) data from 9 independent experiments are given. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24956170), licensed under a CC-BY license. Not internally tested by R&D Systems.

Block/ Neutralize Detection of Human IL-10 by Block/Neutralize View Larger

Detection of Human IL-10 by Block/Neutralize S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha, IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or absence of anti-IL-10 antibodies (Ab). Anti IL-10 Ab was used at 100 ng/ml. The mRNA level was normalized to that of the reference gene peptidylpropylisomerase B (PPIB) and then compared to the control group. Black columns represent controls (cells without any lipopolysaccharide (LPS)), white columns represent LPS-unprimed cells (only stimulated once with 100 ng/ml LPS) and grey columns represent LPS-primed cells (stimulated twice: 2 ng/ml followed by 100 ng/ml). †p<0.01, Wilcoxon paired test. Median (+/− interquartile range) data from 9 independent experiments are given. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24956170), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Human IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Interleukin-10 haplotype may predict survival and relapse in resected non-small cell lung cancer.
    Authors: Wang YC, Sung WW, Wu TC, Wang L, Chien WP, Cheng YW, Chen CY, Shieh SH, Lee H
    PLoS ONE, 2012-07-27;7(7):e39525.
  2. Defective Induction of IL-27-Mediated Immunoregulation by Myeloid DCs in Multiple Sclerosis
    Authors: von Glehn, F;Pochet, N;Thapa, B;Raheja, R;Mazzola, MA;Jangi, S;Beynon, V;Huang, J;Farias, AS;Paul, A;Santos, LMB;Gandhi, R;Murugaiyan, G;Weiner, HL;Baecher-Allan, CM;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells
    Authors: MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani
    Sci Rep, 2019-07-10;9(1):10007.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes
    Authors: Hongxiao Song, Guangyun Tan, Yang Yang, An Cui, Haijun Li, Tianyang Li et al.
    The Journal of Immunology
  5. Oral fibroblasts modulate the macrophage response to bacterial challenge
    Authors: R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum
    Sci Rep, 2017-09-14;7(1):11516.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
    Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
    Arthritis Res. Ther., 2017-05-16;19(1):97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNgamma.
    Authors: Fontaine M, Planel S, Peronnet E, Turrel-Davin F, Piriou V, Pachot A, Monneret G, Lepape A, Venet F
    PLoS ONE, 2014-06-23;9(6):e100909.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.
    Authors: Planes R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E
    J Virol, 2014-04-02;88(12):6672-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. IFN-gamma-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.
    Authors: Svajger U, Obermajer N, Jeras M
    J Leukoc Biol, 2013-08-07;95(1):33-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012-07-03;7(7):e39871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function.
    Authors: Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M
    J. Leukoc. Biol., 2011-03-29;89(6):893-905.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Transcriptional Control of Cytokine Release from Monocytes of the Newborn: Effects of Endogenous and Exogenous Interleukin-10 versus Dexamethasone
    Authors: Lina A. Chusid, Lucy Pereira-Argenziano, Veronika Miskolci, Ivana Vancurova, Dennis Davidson
    Neonatology
  13. Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
    Authors: Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM
    J. Immunol., 2009-03-01;182(5):2929-38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008-04-01;38(4):1012-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo.
    Authors: Hajishengallis G, Shakhatreh MA, Wang M, Liang S
    J. Immunol., 2007-08-15;179(4):2359-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. Splenic accumulation of IL-10 mRNA in T cells distinct from CD4+CD25+ (Foxp3) regulatory T cells in human visceral leishmaniasis
    Authors: Susanne Nylén, Radheshyam Maurya, Liv Eidsmo, Krishna Das Manandhar, Shyam Sundar, David Sacks
    The Journal of Experimental Medicine
  17. Effect of IL-21 on NK cells derived from different umbilical cord blood populations.
    Authors: Perez S, Mahaira L, Sotiropoulou P, Gritzapis A, Iliopoulou E, Niarchos D, Cacoullos N, Kavalakis Y, Antsaklis A, Sotiriadou N, Baxevanis C, Papamichail M
    Int Immunol, 2005-11-15;18(1):49-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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