Human HOXB7 Antibody

Catalog #: MAB8040 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8040
MAB8040-SP

1 Image

All HOXB7 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HOXB7 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HOXB7 in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 864614

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human HOXB7
Met1-Asn123
Accession # P09629

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

View Larger

HOXB7 in MCF‑7 Human Cell Line. HOXB7 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HOXB7 Monoclonal Antibody (Catalog # MAB8040) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOXB7

HOXB7 (Homeobox protein B7), also called Hox-2C, is a 24 kDa (predicted) member of the Antp homeobox family of transcription factors. It is widely expressed and is overexpressed in many cancers such as melanoma and tumors in lung and breast. It promotes tumorigenesis by promoting activities such as proliferation, invasion, epithelial-mesenchymal transition and angiogenesis. Human HOXB7 is a 217 amino acid (aa) protein with an Antp-type hexapeptide (aa 126-131) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 137-196). Human HOXB7 aa 1-123 shares 92% aa sequence identity with mouse and rat HOXB7.

Long Name

Homeobox B7

Entrez Gene IDs

3217 (Human); 15415 (Mouse); 497985 (Rat)

Alternate Names

HHO.C1; homeo box 2C; homeo box B7; homeo box c1 protein; homeobox B7; Homeobox protein HHO.C1; Homeobox protein Hox-2C; homeobox protein Hox-B7; HOX2; Hox-2.3; HOX2C; HOX2CHox-2.3; HOXB7