Human Hormone-sensitive Lipase/HSL Antibody

Catalog # Availability Size / Price Qty
MAB7104
MAB7104-SP
Detection of Human and Mouse Hormone-sensitive Lipase/HSL by Western Blot.
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Product Details
Citations (1)
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Human Hormone-sensitive Lipase/HSL Antibody Summary

Species Reactivity
Human
Specificity
Detects human Hormone-sensitive Lipase/HSL in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 729923
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Hormone-sensitive Lipase/HSL
Met302-Leu425
Accession # Q05469
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Hormone-sensitive Lipase/HSL antibody by Western Blot. View Larger

Detection of Human and Mouse Hormone-sensitive Lipase/HSL by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, NTera-2 human testicular embryonic carcinoma cell line, SVEC4-10 mouse vascular endothelial cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody (Catalog # MAB7104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Hormone-sensitive Lipase/HSL at approximately 70 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Hormone-sensitive Lipase/HSL antibody in Human Testis by Immunohistochemistry (IHC-P). View Larger

Hormone-sensitive Lipase/HSL in Human Testis. Hormone-sensitive Lipase/HSL was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody (Catalog # MAB7104) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hormone-sensitive Lipase/HSL

HSL (hormone-sensitive lipase), also called LIPE, is an 84 kDa phosphoprotein that is the rate-limiting enzyme in the catalytic breakdown of triglycerides. The human 775 amino acid (aa) form is active in adipose tissue and skeletal muscle. Within the region used as an immunogen, human HSL shares 92% and 93% aa sequence identity with mouse and rat HSL, respectively. HSL translocates to triglyceride-metabolizing lipid droplets in response to epinephrine or contraction in skeletal muscle, but to the cytosol following insulin treatment of adipocytes. A 120 kDa isoform of human HSL with a 301 aa N-terminal extension is present in the testes, localized to elongating spermatids and spermatozoa.

Entrez Gene IDs
3991 (Human); 16890 (Mouse); 25330 (Rat)
Alternate Names
hormone-sensitive lipase testicular isoform; Hormonesensitive Lipase; Hormone-sensitive Lipase; HSL; HSLEC 3.1.1.79; LHS; lipase, hormone-sensitive; LIPE

Product Datasheets

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Citation for Human Hormone-sensitive Lipase/HSL Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Loss of ephrin B2 receptor (EPHB2) sets lipid rheostat by regulating proteins DGAT1 and ATGL inducing lipid droplet storage in prostate cancer cells
    Authors: Alejandro Morales, Max Greenberg, Francesca Nardi, Victoria Gil, Simon W. Hayward, Susan E. Crawford et al.
    Laboratory Investigation

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