Human HNF-4 alpha /NR2A1 Antibody

Catalog #: AF4605 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4605
AF4605-SP

Detection of Human HNF‑4 alpha /NR2A1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HNF-4 alpha /NR2A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HNF‑4 alpha /NR2A1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HNF‑4 alpha /NR2A1
Val130-Ser330
Accession # P41235

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

50 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HNF‑4 alpha /NR2A1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human HNF-4a/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band for HNF-4a/NR2A1 was detected at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human HNF‑4 alpha /NR2A1 by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HNF-4a/NR2A1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human HNF-4a/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HNF-4 alpha/NR2A1

HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF-4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Entrez Gene IDs

3172 (Human)

Alternate Names

FLJ39654; hepatic nuclear factor 4 alpha; hepatocyte nuclear factor 4, alpha; hepatocyte nuclear factor 4-alpha; HNF4 alpha; HNF-4 alpha; HNF4A; HNF4a7; HNF-4-alpha; HNF4alpha10/11/12; HNF4HNF4a8; MODY; MODY1; NR2A1; NR2A1HNF4a9; NR2A21; Nuclear receptor subfamily 2 group A member 1; TCF; TCF14; TCF-14; TCF14HNF4alpha; Transcription factor 14; Transcription factor HNF-4; transcription factor-14