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Human HM74A/PUMA-G/GPR109A Antibody

Catalog #: MAB27601 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB27601-100
MAB27601-SP
HM74A/PUMA-G/GPR109A in Human Spleen Tissue.
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Human HM74A/PUMA-G/GPR109A Antibody Summary

Species Reactivity
Human
Specificity
Detects human HM74A/PUMA-G/GPR109A in direct ELISAs.
Source
Monoclonal Rat IgG2B Clone # 245115
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human HM74A/PUMA-G/GPR109A
Met1-Pro363
Accession # Q8TDS4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HM74A/PUMA-G/GPR109A antibody in Human Spleen Tissue by Immunohistochemistry (IHC-P). View Larger

HM74A/PUMA-G/GPR109A in Human Spleen Tissue. HM74A/PUMA-G/GPR109A was detected in immersion fixed paraffin-embedded sections of human spleen tissue using Rat Anti-Human HM74A/PUMA-G/GPR109A Monoclonal Antibody (Catalog # MAB27601) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HM74A/PUMA-G/GPR109A

HM74A, also known as GPR109A, is a high affinity G-protein coupled receptor for nicotinic acid. It is primarily expressed in adipose tissue and spleen.

Long Name
G Protein-coupled Receptor HM74A/G Protein-coupled Receptor 109A
Entrez Gene IDs
338442 (Human); 80885 (Mouse)
Alternate Names
GPR109A; HCA2; HCAR2; HM74A; hydroxycarboxylic acid receptor 2; Niacr1; Nicotinic Acid Receptor; PUMAG

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