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Human HGFR/c-MET Antibody

Catalog #: MAB3582
14 Citations 4 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3582
MAB3582-SP
Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
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Product Details
Citations (14)
FAQs
Supplemental Products
Reviews (4)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HGFR/c-MET Antibody Summary

Species Reactivity
Human
Specificity
Detects human HGF R/c-MET.
Source
Monoclonal Mouse IgG1 Clone # 95106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET
Glu25-Thr932
Accession # P08581
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of HGF R/c‑MET antibody in MDA‑MB‑231 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of HGF R/c‑MET in MDA‑MB‑231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast cancer cell line was stained with Human HGF R/c-MET Monoclonal Antibody (Catalog # MAB3582, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGFR/c-MET

HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain (1, 2). The extracellular domain (ECD) contains a seven bladed beta -propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain (3, 4). Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains (5-7). The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization (3, 8). Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules (9, 10). HGF stimulation induces HGF R downregulation via internalization and proteasome-dependent degradation (11). In the absence of ligand, HGF R forms non-covalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/ beta 4, Plexins B1, 2, 3, and MSP R/Ron (12-19). Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects (12-19). Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion (12, 16, 17). Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells (20). Genetic polymorphisms, chromosomal translocation, over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers (1). Within the ECD, human HGF R shares 86-88% amino acid sequence identity with canine, mouse, and rat HGF R.

References
  1. Birchmeier, C. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:915.
  2. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  3. Gherardi, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100:12039.
  4. Park, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:6379.
  5. Crepaldi, T. et al. (1994) J. Biol. Chem. 269:1750.
  6. Prat, M. et al. (1991) Mol. Cell. Biol. 12:5954.
  7. Rodrigues, G.A. et al. (1991) Mol. Cell. Biol. 11:2962.
  8. Kong-Beltran, M. et al. (2004) Cancer Cell 6:75.
  9. Naldini, L. et al. (1991) Mol. Cell. Biol. 11:1793.
  10. Ponzetto, C. et al. (1994) Cell 77:261.
  11. Jeffers, M. et al. (1997) Mol. Cell. Biol. 17:799.
  12. Orian-Rousseau, V. et al. (2002) Genes Dev. 16:3074.
  13. Klosek, S.K. et al. (2005) Biochem. Biophys. Res. Commun. 336:408.
  14. Jo, M. et al. (2000) J. Biol. Chem. 275:8806.
  15. Wang, X. et al. (2002) Mol. Cell 9:411.
  16. Trusolino, L. et al. (2001) Cell 107:643.
  17. Giordano, S. et al. (2002) Nat. Cell Biol. 4:720.
  18. Conrotto, P. et al. (2004) Oncogene 23:5131.
  19. Follenzi, A. et al. (2000) Oncogene 19:3041.
  20. Sonnenberg, E. et al. (1993) J. Cell Biol. 123:223.
Long Name
Hepatocyte Growth Factor Receptor
Entrez Gene IDs
4233 (Human); 17295 (Mouse)
Alternate Names
AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

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Citations for Human HGFR/c-MET Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. c-Met-Specific Chimeric Antigen Receptor T Cells Demonstrate Anti-Tumor Effect in c-Met Positive Gastric Cancer
    Authors: CH Kang, Y Kim, DY Lee, SU Choi, HK Lee, CH Park
    Cancers, 2021-11-16;13(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Aberrant Expression of and Cell Death Induction by Engagement of the MHC-II Chaperone CD74 in Anaplastic Large Cell Lymphoma (ALCL)
    Authors: KD Wurster, M Costanza, S Kreher, S Glaser, B Lamprecht, N Schleussne, I Anagnostop, M Hummel, K Jöhrens, H Stein, A Molina, A Diepstra, B Gillissen, K Köchert, R Siebert, O Merkel, L Kenner, M Janz, S Mathas
    Cancers, 2021-10-07;13(19):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Adipose stem cell niche reprograms the colorectal cancer stem cell metastatic machinery
    Authors: S Di Franco, P Bianca, DS Sardina, A Turdo, M Gaggianesi, V Veschi, A Nicotra, LR Mangiapane, M Lo Iacono, I Pillitteri, S van Hooff, F Martorana, G Motta, E Gulotta, VL Lentini, E Martorana, ME Fiori, S Vieni, MR Bongiorno, G Giannone, D Giuffrida, L Memeo, L Colarossi, M Mare, P Vigneri, M Todaro, R De Maria, JP Medema, G Stassi
    Nature Communications, 2021-08-18;12(1):5006.
    Species: Human
    Sample Types: Whole Tissue
    Applications: ICC
  4. ERBB3 overexpression due to miR-205 inactivation confers sensitivity to FGF, metabolic activation, and liability to ERBB3 targeting in glioblastoma
    Authors: F De Bacco, F Orzan, J Erriquez, E Casanova, L Barault, R Albano, A D'Ambrosio, V Bigatto, G Reato, M Patanè, B Pollo, G Kuesters, C Dell'Aglio, L Casorzo, S Pellegatta, G Finocchiar, PM Comoglio, C Boccaccio
    Cell Reports, 2021-07-27;36(4):109455.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer
    Authors: T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi
    Oncol Rep, 2020-10-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma
    Authors: BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd
    Sci Rep, 2019-03-20;9(1):4902.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. TGF beta 1 regulates HGF ‐induced cell migration and hepatocyte growth factor receptor MET expression via C‐ets‐1 and miR‐128‐3p in basal‐like breast cancer
    Authors: Christian Breunig, Nese Erdem, Alexander Bott, Julia F. Greiwe, Eileen Reinz, Stephan Bernhardt et al.
    Molecular Oncology
  8. Necrosis- and apoptosis-related Met cleavages have divergent functional consequences
    Authors: R Montagne, M Berbon, L Doublet, N Debreuck, A Baranzelli, H Drobecq et al.
    Cell Death & Disease
  9. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
    Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
    Cell Death Differ, 2015-01-09;22(7):1219-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. beta1 integrin targeting potentiates antiangiogenic therapy and inhibits the growth of bevacizumab-resistant glioblastoma.
    Authors: Carbonell W, DeLay M, Jahangiri A, Park C, Aghi M
    Cancer Res, 2013-05-03;73(10):3145-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell lines
    Authors: Ling Xu, Martin Hausmann, Wolfgang Dietmaier, Silvia Kellermeier, Theresa Pesch, Manuela Stieber-Gunckel et al.
    BMC Cancer
  12. Down-regulation of the met receptor tyrosine kinase by presenilin-dependent regulated intramembrane proteolysis.
    Authors: Foveau B, Ancot F, Leroy C, Petrelli A, Reiss K, Vingtdeux V, Giordano S, Fafeur V, Tulasne D
    Mol. Biol. Cell, 2009-03-18;20(9):2495-507.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
    Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
    Leuk. Res., 2008-03-04;32(8):1244-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. H1/pHGFK1 nanoparticles exert anti-tumoural and radiosensitising effects by inhibition of MET in glioblastoma
    Authors: W Zhang, R Duan, J Zhang, WKC Cheung, X Gao, R Zhang, Q Zhang, M Wei, G Wang, Q Zhang, PJ Mei, HL Chen, H Kung, MC Lin, Z Shen, J Zheng, L Zhang, H Yao
    Br. J. Cancer, 2018-01-18;0(0):.

FAQs

  1. Does Human HGFR/c-MET Antibody, Catalog # MAB3582, bind to the alpha chain or beta chain of HGFR/c-MET?

    • The immunogen for MAB3582 (Recombinant human HGF R/c­MET, aa Glu25­-Thr932) spans both the alpha and beta chain regions of Human HGFR and we do not epitope map our antibodies. However, staining in flow cytometry detects a surface epitope. Since only the alpha chain is extracellular in location and the beta chain is transmembrane in location, the antibody likley binds the alpha chain of this protein.

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Reviews for Human HGFR/c-MET Antibody

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Human HGFR/c-MET Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human HGFR/c-MET Antibody MAB3582
Human HGFR/c-MET Antibody
By Anonymous on 12/23/2020
Application: Flow Sample Tested: A-431 epidermoid carcinoma cell line Species: Human

Detection of human HGFR/c-MET on epidermoid carcinoma cell line A-431. A-431 cells were treated with 100 nM of the human HGFR/c-MET antibody (catalog # MAB3582) or mouse IgG1 isotype control, followed by a secondary antibody goat a-mouse IgG Fc APC. Isotype control (RED), human HGFR/c-MET antibody (BLUE).

Flow Cytometry Human HGFR/c-MET Antibody MAB3582
Human HGFR/c-MET Antibody
By Anonymous on 02/08/2020
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma

Microarray Human HGFR/c-MET Antibody MAB3582
Human HGF R/c-MET Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human