Human HES-4 Biotinylated Antibody

Catalog #: BAF3600 Datasheet / COA / SDS

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BAF3600

All HES-4 Antibodies

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Human HES-4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HES-4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) HES-1, rhHES-2, rhHES-5, and rhHES-7 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HES-4
Val94-Arg221
Accession # Q9HCC6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human HES‑4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HES-4

HES-4 is a 221 aa, 24 kDa member of the HES-family of transcriptional repressors. HES-4 contains a species-specific bHLH domain (aa 33‑93), a specific orange DNA-binding domain (aa 109‑150), a 65 aa Proline-rich SH3-binding domain, and a WRPW Groucho/TLE interacting C-terminal motif. HES-4 is a HES-1 paralog likely to maintain stem cell pluripotency. In the region used as immunogen, the amino acid sequence of human and canine HES-4 are 80% identical.

Long Name

Hairy And Enhancer of Split 4

Entrez Gene IDs

57801 (Human)

Alternate Names

bHLH factor Hes4; BHLHB42; bHLHb42transcription factor HES-4; Class B basic helix-loop-helix protein 42; hairy and enhancer of split 4 (Drosophila); Hairy and enhancer of split 4; HES4; HES-4; hHES4