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Human HAI‑2 Ectodomain Antibody

Catalog #: MAB1106
2 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1106
MAB1106-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (3)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HAI‑2 Ectodomain Antibody Summary

Species Reactivity
Human
Specificity
Detects recombinant human (rh)HAI-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhHAI-1 or rmHAI-2B.
Source
Monoclonal Mouse IgG1 Clone # 170018
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-2
aa 28-197
Accession # O43291
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human HAI‑2 (Catalog # 1106-PI)

Human HAI-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human HAI‑2 Biotinylated Antibody (Catalog # BAF1106)

Standard: Recombinant Human HAI-2A Protein, CF (Catalog # 1106-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2

HGF Activator Inhibitor type II (HAI-2), also known as placental bikunin, is a Kunitz-type protease inhibitor. It is a type I membrane protein containing 2 Kunitz inhibitor domains in its extracellular region. In mouse, at least three isoforms of HAI-2 exist as a result of alternative splicing. Isoforms 2 and 3, also known as HAI-2B and HAI-2C, respectively, are both missing the first amino-terminal Kunitz inhibitor domain.

Long Name
HGF Activator Inhibitor Type 2
Entrez Gene IDs
10653 (Human); 20733 (Mouse)
Alternate Names
HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2

Product Datasheets

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Citations for Human HAI‑2 Ectodomain Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Epigenetic Silencing of SPINT2 Promotes Cancer Cell Motility via HGF-MET Pathway Activation in Melanoma.
    Authors: Hwang S, Kim H, Min M, Raghunathan R, Panova I, Munshi R, Ryu B
    J Invest Dermatol, 2015-04-24;135(9):2283-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. An epigenetic genome-wide screen identifies SPINT2 as a novel tumor suppressor gene in pediatric medulloblastoma.
    Authors: Kongkham PN, Northcott PA, Ra YS
    Cancer Res., 2008-12-01;68(23):9945-53.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Reviews for Human HAI‑2 Ectodomain Antibody

Average Rating: 3.7 (Based on 3 Reviews)

5 Star
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4 Star
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3 Star
33.33%
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Human HAI‑2 Ectodomain Antibody
By Anonymous on 01/14/2021
Application: Microarrays Sample Tested: EDTA Plasma Species: Human
Other Human HAI‑2 Ectodomain Antibody MAB1106
Human HAI‑2 Ectodomain Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human
Human HAI‑2 Ectodomain Antibody
By Anonymous on 11/14/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human