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Human HAI-2 Antibody

Catalog #: AF1106
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1106
AF1106-SP
Product Details
Citations (4)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HAI-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HAI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human HAI‑1 and recombinant mouse HAI-2B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAI-2A
Ala28-Lys197
Accession # O43291
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HAI‑2A (Catalog # 1106-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human HAI‑2A (Catalog # 1106‑PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2

Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1-5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa. Recombinant HAI-2A prolonged the clotting time in an activated partial thromboplastin time assay.

References
  1. Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
  2. Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558.
  3. Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202.
  4. Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88.
  5. Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.
Long Name
HGF Activator Inhibitor Type 2
Entrez Gene IDs
10653 (Human); 20733 (Mouse)
Alternate Names
HAI2; HAI-2; HAI-2DIAR3; HAI2FLJ45571; Hepatocyte growth factor activator inhibitor type 2; Kop; kunitz-type protease inhibitor 2; PB; Placental bikunin; serine peptidase inhibitor, Kunitz type, 2; serine protease inhibitor, Kunitz type, 2

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Citations for Human HAI-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Exosome-Mediated Activation of the Prostasin-Matriptase Serine Protease Cascade in B Lymphoma Cells
    Authors: Li-Mei Chen, Karl X. Chai
    Cancers (Basel)
  2. Post-translational modifications of serine protease TMPRSS13 regulate zymogen activation, proteolytic activity, and cell surface localization
    Authors: CE Martin, AS Murray, KE Sala-Hamri, JR Mackinder, EC Harrison, JG Lundgren, FA Varela, K List
    The Journal of Biological Chemistry, 2021-09-22;0(0):101227.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization
    Authors: AS Murray, FA Varela, TE Hyland, AJ Schoenbeck, JM White, LM Tanabe, SV Todi, K List
    J. Biol. Chem., 2017-07-14;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Targeting the membrane-anchored serine protease testisin with a novel engineered anthrax toxin prodrug to kill tumor cells and reduce tumor burden
    Authors: Erik W. Martin, Marguerite S. Buzza, Kathryn H. Driesbaugh, Shihui Liu, Yolanda M. Fortenberry, Stephen H. Leppla et al.
    Oncotarget

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