Human GSK-3 beta Antibody

Catalog #: MAB25061
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB25061
MAB25061-SP

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All GSK-3 beta Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GSK-3 beta Antibody Summary

Species Reactivity

Human

Specificity

Detects human GSK-3 beta.

Source

Monoclonal Rat IgG_2B Clone # 272535

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GSK‑3 beta in MCF-7 Human Cell Line. GSK‑3 beta was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GSK‑3 beta Monoclonal Antibody (Catalog # MAB25061) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved inenergy metabolism, body pattern formation, and neuronal cell development.

Long Name

Glycogen Synthase Kinase 3 beta

Entrez Gene IDs

2932 (Human); 56637 (Mouse); 84027 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human GSK-3 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Discovery of novel AKT inhibitors with enhanced anti-tumor effects in combination with the MEK inhibitor.
Authors: Dumble, Melissa, Crouthamel, Ming-Chi, Zhang, Shu-Yun, Schaber, Michael, Levy, Dana, Robell, Kimberly, Liu, Qi, Figueroa, David J, Minthorn, Elisabet, Seefeld, Mark A, Rouse, Meagan B, Rabindran, Sridhar, Heerding, Dirk A, Kumar, Rakesh
PLoS ONE, 2014-06-30;9(6):e100880.
Species: Human
Sample Types: Whole Cells
Applications: ELISA Development